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| * '''Description:''' sporulation initiation phosphotransferase of the [[phosphorelay]]<br/><br/>
| | {{#bank: Spo0B}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spo0B''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spo0D ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || sporulation initiation phosphotransferase <br/>
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| of the [[phosphorelay]]
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| |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of sporulation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27930 spo0B]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/Spo0B Spo0B]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 4.963
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 576 bp, 192 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[obg]]'', ''[[rpmA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27930 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spo0B_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spo0B_2853981_2854559_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:spo0B_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27930]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[phosphorelay]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU27930
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| ===Phenotypes of a mutant ===
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| Arrest of sporulation at stage 0 (initiation) [http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=4633157 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/spo0B-obg.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10336]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[Spo0B]]-[[Spo0F]]
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| ** [[Spo0B]]-[[Spo0A]]
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| ** [[YmcA]]-[[Spo0F]] {{PubMed|23490197}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1F51 1F51] (complex with [[Spo0F]]), [http://www.rcsb.org/pdb/explore.do?structureId=1IXM 1IXM]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P06535 P06535]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27930]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[spo0B]]-[[obg]]'' {{PubMed|2537815}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=spo0B_2853981_2854559_-1 spo0B] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2537815}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| **[http://www.ncbi.nlm.nih.gov/nuccore/254763670 spo0B12], point mutation 85C>T (=R29W), available in [http://www.bgsc.org/ BGSC] 1S54
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| **[http://www.ncbi.nlm.nih.gov/nuccore/254763672 spo0B136], amber mutation 103A>T (=K35X), available in [http://www.bgsc.org/ BGSC] 1S16
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| **[http://www.ncbi.nlm.nih.gov/nuccore/254763674 spo0B580ts], point mutation 193G>A (=E65K), available in [http://www.bgsc.org/ BGSC] 1S90
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| **[http://www.ncbi.nlm.nih.gov/nuccore/254763676 spo0B581ts], point mutation 461G>A (=G154D), available in [http://www.bgsc.org/ BGSC] 1S91
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>20413551 12730135,2437099,9141138,3918016,9299348,16788205,12067336,9726997,2118512, 10997904,1846779,9477965, 2537815 23490197</pubmed>
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| [[Category:Protein-coding genes]]
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