|
|
| (6 intermediate revisions by 3 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' [[RNA polymerase]] forespore-specific (early) [[sigma factor]] SigF <br/><br/>
| | {{#bank: SigF}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''sigF''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIAC ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || [[RNA polymerase]] forespore-specific <br/>(early) [[sigma factor]] SigF
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || [[transcription]] of sporulation genes (early forespore)
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23450 sigF]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SigF SigF]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.078
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoVAA]]'', ''[[spoIIAB]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23450 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23450 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23450 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:sigF_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigF_2443429_2444196_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:sigF_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23450]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[transcription]]}},
| |
| {{SubtiWiki category|[[sigma factors and their control]]}},
| |
| {{SubtiWiki category|[[sporulation proteins]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[AbrB regulon]]}},
| |
| {{SubtiWiki regulon|[[SigF regulon]]}},
| |
| {{SubtiWiki regulon|[[SigG regulon]]}},
| |
| {{SubtiWiki regulon|[[SigH regulon]]}},
| |
| {{SubtiWiki regulon|[[SinR regulon]]}},
| |
| {{SubtiWiki regulon|[[Spo0A regulon]]}}
| |
|
| |
| =The [[SigF regulon]]=
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU23450
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacF-spoIIAAB-sigF.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10298]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
|
| |
| * '''Protein family:''' sigma-70 factor family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):''' [[SigG]]
| |
|
| |
| === Genes controlled by SigF ===
| |
|
| |
| ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[sigG]]'','' [[pbpF]]'', ''[[yphA]]-[[seaA]]''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[SigF]]-[[Fin]] (probably) {{PubMed|21037003}}
| |
| ** [[SigF]]-[[SpoIIAB]]
| |
| ** [[SigF]]-([[RpoB]]-[[RpoC]])
| |
|
| |
| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1L0O 1L0O] (complex with SpoIIAB, Geobacillus stearothermophilus)
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P07860 P07860]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23450]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
| ** ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
| |
| ** ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=sigF_2443429_2444196_-1 sigF] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
| ** ''[[dacF]]'': [[SigF]], [[SigG]]
| |
| ** ''[[spoIIAA]]'': [[SigH]]
| |
|
| |
| * '''Regulation:'''
| |
| ** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]
| |
| ** ''[[dacF]]'': expressed during sporulation
| |
| ** ''[[spoIIAA]]'': expressed early during sporulation
| |
| ** expressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]]
| |
| ** [[Spo0A]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/1556084,15687200 PubMed]
| |
| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| * [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed>20833318 </pubmed>
| |
| ==The [[SigF regulon]]==
| |
| <pubmed>16497325</pubmed>
| |
| ==Original Publications==
| |
| '''Additional publications:''' {{PubMed|23169620,21037003,20817675,21935351,22312331}}
| |
| <pubmed>10503549,8358793,7570023,9555886,3007937,8460142,9077448,10323866,10869437,7768847,14744853,8759874,10864495,1744043,16824103,11701124,1902463,8171000,1904527,15351644,15882622,8764398,8764397,9826498,9826499,2492512,1629150,12676949,7592342,11684022,8846916,1948031,8126438, 3114419,12081956,9150232,15205443,9004507,8168129,2123551,8824593,19390092,17498739,8021191,1556084,15687200,12107147, 20298743 18208527 10049355</pubmed>
| |
| [[Category:Protein-coding genes]]
| |
