|
|
| (2 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' sporulation-specific [[penicillin-binding protein]] 5, D-alanyl-D-alanine carboxypeptidase <br/><br/>
| | {{#bank: DacB}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''dacB''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || [[penicillin-binding protein]] 5, D-alanyl-D-alanine carboxypeptidase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || carboxypeptidase
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23190 dacB]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.725
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1146 bp, 382 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spmA]]'', ''[[ypuI]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23190 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23190 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23190 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:dacB_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dacB_2423383_2424531_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:dacB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU23190]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[cell wall synthesis]]}},
| |
| {{SubtiWiki category|[[sporulation proteins]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[SigE regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU23190
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacB-spmAB.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10527]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' modifies degree of cross-linking of glycan strands in peptidoglycan
| |
|
| |
| * '''Protein family:''' peptidase S11 family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** cell membrane (according to Swiss-Prot)
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=3MFD 3MFD] (beta-lactamase superfamily domain)
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P35150 P35150]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23190]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''[[dacB]]-[[spmA]]-[[spmB]]'' {{PubMed|7642500}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dacB_2423383_2424531_-1 dacB] {{PubMed|22383849}}
| |
|
| |
| * '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190,7528199}}
| |
|
| |
| * '''Regulation:'''
| |
| ** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|15699190,7528199}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
| ** 1A744 ( ''dacB''::''cat''), {{PubMed|1644769}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A744&Search=1A744 BGSC]
| |
| ** 1A745 ( ''dacB''::''cat''), {{PubMed|1644769}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A745&Search=1A745 BGSC]
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| <pubmed>10498740,9864321,1548223,7642500,14731276,19542328 ,7528199 15699190 10383963 22123250</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
