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| * '''Description:''' translation initiation factor IF-2 <br/><br/>
| | {{#bank: InfB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''infB''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || initiation factor IF-2
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| |style="background:#ABCDEF;" align="center"|'''Function''' || translation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16630 infB]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 78 kDa, 5.191
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2148 bp, 716 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ylxQ]]'', ''[[ylxP]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16630 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16630 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16630 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:infB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=infB_1734009_1736159_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:infB_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16630]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16630
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ylxS-nusA-ylxRQ-infB-ylxP-rbfA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10268]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Initiation of translation
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| * '''Protein family:''' IF-2 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:''' Nucleotide-binding domain that competitively binds GTP or ppGpp {{PubMed|16968770}}
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' GTP and ppGpp {{PubMed|16968770}}
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1D1N 1D1N] (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=1Z9B 1Z9B] (C1-subdomain, Geobacillus stearothermophilus)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P17889 P17889]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16630]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ylxS]]-[[nusA]]-[[ylxR]]-[[ylxQ]]-[[infB]]-[[ylxP]]-[[rbfA]]'' {{PubMed|8491709}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=infB_1734009_1736159_1 infB] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|8491709}}
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| * '''Regulation:'''
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| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
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| ** Interaction with ppGpp interferes with IF2-dependent initiation complex formation, severely inhibits initiation dipeptide formation, and blocks the initiation step of translation.{{PubMed|16968770}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:''' Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients {{PubMed|16968770}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>11948165 8491709 16968770</pubmed>
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| [[Category:Protein-coding genes]]
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