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| * '''Description:''' fructose-specific [[PTS|phosphotransferase system]], EIIA component of the [[PTS]]<br/><br/>
| | {{#bank: LevD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''levD''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || fructose-specific [[PTS|phosphotransferase system]], <br/>EIIA component
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| |style="background:#ABCDEF;" align="center"|'''Function''' || fructose uptake and phosphorylation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27070 levD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/LevD LevD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 16 kDa, 4.479
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 438 bp, 146 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[levE]]'', ''[[levR]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27070 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27070 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27070 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:levD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levD_2762395_2762835_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:levD_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[phosphotransferase systems]]}},
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| {{SubtiWiki category|[[utilization of specific carbon sources]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[LevR regulon]]}},
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| {{SubtiWiki regulon|[[SigL regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU27070
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10316]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine (according to Swiss-Prot)
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| * '''Protein family:''' [[PTS]] permease, mannose permease (Man) family [http://www.ncbi.nlm.nih.gov/pubmed/10627040 PubMed]
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[LevD]]-[[PtsH|HPr]], [[LevD]]-[[LevE]]
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P26379 P26379]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27070]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2117666 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=levD_2762395_2762835_-1 levD] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigL]] {{PubMed|1924373}}
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| * '''Regulation:'''
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| ** subject to carbon catabolite repression ([[CcpA]], [[LevR]]) {{PubMed|7592486}}
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| ** induced in the presence of fructose ([[LevR]]) {{PubMed|1900939}}
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| * '''Regulatory mechanism:'''
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| ** [[CcpA]]: transcription repression {{PubMed|7592486}}
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| ** [[LevR]]: transcription activation {{PubMed|1900939}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>10627040 2117666 1619665 7592487 7592486 9033408, 1924373 7592486 1900939 22900538 </pubmed>
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| [[Category:Protein-coding genes]]
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