|
|
| (4 intermediate revisions by 3 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' pyrroline-5-carboxylate reductase <br/><br/>
| | {{#bank: ProH}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''proH''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yoxE ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || pyrroline-5-carboxylate reductase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || osmoadaptive de novo production of proline
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU18480 proH]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/proline.html Pro], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 7.754
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 891 bp, 297 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[proJ]]'', ''[[rtp]]''
| |
| |-
| |
| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU18480 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU18480 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU18480 DNA_with_flanks]
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:proH_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proH_2016845_2017738_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:proH_expression.png|500px]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
|
| |
|
| |
| <br/><br/><br/><br/><br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}},
| |
| {{SubtiWiki category|[[coping with hyper-osmotic stress]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
|
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU18480
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' no entry
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11049]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' L-proline + NAD(P)<sup>+</sup> = 1-pyrroline-5-carboxylate + NAD(P)H (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' glutamate 5-kinase family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):''' [[ProI]]
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
|
| |
| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P14383 P14383]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU18480]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.5.1.2 1.5.1.2]
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' ''[[proH]]-[[proJ]]'' {{PubMed|21296969}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proH_2016845_2017738_-1 proH] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:''' [[SigA]] {{PubMed|21784929}}
| |
|
| |
| * '''Regulation:'''
| |
| ** expressed under conditions of osmotic stress {{PubMed|21784929}}
| |
| ** induced by cold stress {{PubMed|21296969}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| '''Additional publications:''' {{PubMed|21784929}}
| |
| <pubmed> ,11418582, 21296969</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
