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| * '''Description:''' [[RNase]] III; required for the degradation of sense/antisense transcripts in Gram positive, low GC bacteria<br/><br/>
| | {{#bank: Rnc}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rnc''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''rncS ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes
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| |style="background:#ABCDEF;" align="center"| '''Product''' || endoribonuclease III
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| |style="background:#ABCDEF;" align="center"|'''Function''' || cleaves both 5'- and 3'-sites <br/>of the small cytoplasmic RNA precursor (''[[scr]]'')
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU15930 rnc]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/wiki/index.php/Protein_secretion Protein secretion]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 8.076
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 747 bp, 249 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[acpA]]'', ''[[smc]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU15930 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU15930 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU15930 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rnc_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnc_1665710_1666459_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:rnc_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[Rnases]]}},
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| {{SubtiWiki category|[[translation]]}},
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| {{SubtiWiki category|[[protein secretion]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[stringent response]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU15930
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| ===Phenotypes of a mutant ===
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| * essential {{PubMed|23300471,12682299,11123676}}
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| * the ''[[rnc]]'' gene can be deleted in strains cured of the [[Skin element]] and [[SPß prophage]] (or upon deletion of the toxin genes ''[[txpA]]'' and ''[[yonT]]'') {{PubMed|23300471}}
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rnc-smc-ftsY.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11537]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** Endonucleolytic cleavage of double-stranded RNA to 5'-phosphomonoester (according to Swiss-Prot)
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| ** cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[scr]]'')
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| ** degradation of ''[[txpA]]'' mRNA-[[RatA]] RNA duplex (encoded on the [[Skin element]]) {{PubMed|23300471}}
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| ** degradation of ''[[yonT]]'' mRNA-''[[as-yonT]]'' RNA duplex (encoded on the [[SP-beta prophage]]) {{PubMed|23300471}}
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P51833 P51833]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU15930]
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| * '''E.C. number:''' 3.1.26.3
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[rnc]]-[[smc]]-[[ftsY]]'' {{PubMed|11021934}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rnc_1665710_1666459_1 rnc] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|11021934}}
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| * '''Regulation:'''
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| ** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[David Bechhofer]], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers%20Laboratories%20and%20Programs/Bechhofer%20Laboratory?citype=Physician&ciid=Bechhofer%20David%20H%201255565 Homepage]
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| [[Ciaran Condon]], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 17194582 16179796 8936814 16855311 23300472 </pubmed>
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| ==Original publications==
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| <big>''Durand S, Gilet L, Condon C'' </big>
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| <big>'''The essential function of ''B. subtilis'' RNase III is to silence foreign toxin genes.''' </big>
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| <big>PLoS Genet. 2012 8(12): e1003181.</big>
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| [http://www.ncbi.nlm.nih.gov/pubmed/23300471 PubMed:23300471]
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| <pubmed>9393702 11123676 7527387 9677377 17576666, 11021934 6409421 22123973 22412379</pubmed>
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| [[Category:Protein-coding genes]]
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