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| * '''Description:''' buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets [[ComK]] to [[ClpC]]-[[ClpP]] degradation machine (in log Phase), inhibits the transcriptional activity of [[Spo0A]]∼P by direct interaction <br/><br/>
| | {{#bank: MecA}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mecA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || adaptor protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[ComK]] degradation, regulation of competence
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU11520 mecA]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MecA MecA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 4.209
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 654 bp, 218 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yjbE]]'', ''[[coiA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU11520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU11520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU11520 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mecA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mecA_1229068_1229724_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mecA_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[genetic competence]]}},
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| {{SubtiWiki category|[[proteolysis]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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|
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU11520
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|
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mecA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10680]
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|
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| === Additional information===
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|
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| =The protein=
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|
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' mecA family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[YpbH]]
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|
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| ** N-terminal domain (1 ... 120): recruitment of protein substrates {{PubMed|19801546}}
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| ** C-terminal domain (121 ... 218): facilitates assembly of the [[ClpC]]-[[ClpP]] oligomer and acts as a degradation tag {{PubMed|19801546}}
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[MecA]]-[[ComK]] {{PubMed|10447896}}
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| ** [[MecA]]-[[ComS]] {{PubMed|10447896}}
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| ** [[ClpC]]-[[MecA]] {{PubMed|10447896}}
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| ** [[MecA]]-[[Spo0A]] {{PubMed|21435029}}
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| * '''[[Localization]]:'''
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|
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| === Database entries ===
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| * '''Structure:'''
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| ** [http://www.proteopedia.org/wiki/index.php/3jtp 3JTP] (C-terminal domain) {{PubMed|19801546}}
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| ** [http://www.rcsb.org/pdb/explore/explore.do?pdbId=3PXG 3PXG] (the [[MecA]]-[[ClpC]] complex) {{PubMed|21368759}}
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|
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37958 P37958]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11520]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[mecA]]'' {{PubMed|8412687}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mecA_1229068_1229724_1 mecA] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|8412687}}
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP813 (spc), GP814 (aphA3) both available in the [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews ==
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| '''Additional reviews:''' {{PubMed|23479438,19609260}}
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| <pubmed> 23375660 </pubmed>
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| ==Original Publications==
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| '''Additonal publications:''' {{PubMed|16525504,12598648,21435029}}
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| <pubmed>11914365,9000055,17560370,19361434, 9890793,2113920,8817496, , 8412687,11918817, 10447896, 12028382, 8016067, 10361283, 11703662 9535081 19767395 19801546 8016066 19202088 8083167 21368759</pubmed>
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| [[Category:Protein-coding genes]]
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