PWH844: Difference between revisions
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pWH844 | [[File:PWH844.jpeg|250px|thumb|right|'''pWH844: click to enlarge''']] | ||
[[File:PWH844_cloning_region.jpeg|250px|thumb|right|'''pWH844 cloning region: click to enlarge''']] | |||
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein | ||
the [[plasmid]] carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | |||
constructed in the [[Hillen]] lab | constructed in the [[Hillen]] lab | ||
in E. coli: ampicillin resistance | in ''E. coli'': ampicillin resistance | ||
similar [[plasmids|plasmid]]: [[pGP570]] | |||
sequencing primer: | |||
*<font color="#ff0000">'''JS39'''</font>: 5’-TCTATCAACAGGAGTCCAAGC-3‘ | |||
*'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | |||
*'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | |||
*<font color="#99E500">'''JK37'''</font>: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘ | |||
<pubmed> 9023219 </pubmed> | |||
== Return to [[Plasmids]] == | |||
Latest revision as of 14:23, 8 October 2013
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- JK37: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘
<pubmed> 9023219 </pubmed>