PAC5: Difference between revisions

PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’

Latest revision as of 12:58, 28 October 2026

pAC5 cloning region: click to enlarge

Vector for the construction of translational lacZ fusions that can be integrated at the amyE site

Does not replicate in B. subtilis, integrates into the amyE site

Constructed in the Rapoport lab

sequencing primers:

@@ Line 1: / Line 1: @@
-vector for
+[[File:PAC5.jpeg|250px|thumb|right|'''pAC5: click to enlarge''']]
-constructed in the --- lab
+[[File:PAC5_cloning_region.jpeg|250px|thumb|right|'''pAC5 cloning region: click to enlarge''']]
-in E. coli: ampicillin resistance
-in B. subtilis: ---- resistance
+Vector for the construction of translational ''lacZ'' fusions that can be integrated at the ''amyE'' site
-based on ---
-reference. PubMed
+Does not replicate in ''B. subtilis'', integrates into the ''amyE'' site
+Constructed in the Rapoport lab
+* in ''E. coli'': ampicillin resistance
+* in ''B. subtilis'': chloramphenicol resistance
+* similar vectors: [[pAC6]], [[pAC7]]
+sequencing primers:
+*'''PAC5R:''' 5’-CTGCAAGCGATAAGTTGG-3’
+*'''PAC5F:''' 5’-GCGTAGCGAAAAATCCTTTTC-3‘
+*'''Catcheckrev:''' 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’
+<pubmed> 1619665 </pubmed>
+==  Return to [[Plasmids]] ==