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| * '''Description:''' DNA mismatch repair (mismatch recognition protein) <br/><br/>
| | {{#bank: MutS}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''mutS''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || mismatch recognition protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU17040 mutS]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/MutS MutS]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 97 kDa, 5.189
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2574 bp, 858 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cotE]]'', ''[[mutL]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13577]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:mutS_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutS_1775745_1778321_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:mutS_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU17040
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/mutSL.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11403]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** MutS recognizes mismatches in the DNA and recruits [[MutL]] to the site of mismatch recognition
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| * '''Protein family:''' DNA mismatch repair mutS family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[MutS]]-[[DnaN]] {{PubMed|20453097}}
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| ** [[DnaE]]-[[MutS]] {{PubMed|21958350}}
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| * '''[[Localization]]:'''
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| ** forms foci at midcell position, the frequency of foci increases upon mismatch formation {{PubMed|21958350}}
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| ** Nucleoid (Mid-cell) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P49849 P49849]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU17040]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[mutS]]-[[mutL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8760914 PubMed]
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=mutS_1775745_1778321_1 mutS] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** An [[ncRNA|antisense RNA]] is predicted for ''[[mutS]]'' {{PubMed|20525796}}
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| =Biological materials =
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| * '''Mutant:''' GP1190 (del ''mutSL''::''aphA3'') available in the [[Stülke]] lab
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| ** 1A833 ( ''mutS''::''spec''), {{PubMed|11779496}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A833&Search=1A833 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|21958350}}
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| <pubmed>8760914, 15375129,16479537 20525796 20453097 23228104 </pubmed>
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| [[Category:Protein-coding genes]]
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