|
|
| (7 intermediate revisions by 3 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' copper-transporting ATPase, resistance to copper <br/><br/>
| | {{#bank: CopA}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''copA''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvgX ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || copper transporting ATPase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || copper export, detoxification
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU33500 copA]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/CopA CopA]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://cellpublisher.gobics.de/view/GqbblRJkBH metal ion homeostasis]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 85 kDa, 5.484
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2409 bp, 803 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cadA]]'', ''[[copZ]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15355]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:yvgX_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=copA_3441121_3443529_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:copA_expression.png|500px]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
|
| |
|
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[transporters/ other]]}},
| |
| {{SubtiWiki category|[[trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)]]}},
| |
| {{SubtiWiki category|[[resistance against toxic metals]]}},
| |
| {{SubtiWiki category|[[membrane proteins]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CsoR regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU33500
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' no entry
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14106]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' ATP + H<sub>2</sub>O + Cu<sup>1+</sup>(In) = ADP + phosphate + Cu<sup>1+</sup>(Out) (according to Swiss-Prot)
| |
|
| |
| * '''Protein family:''' Type IB subfamily (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
| ** two N-terminal soluble domains, CopAa and CopAb, connected by a short linker
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[CopA]]-[[CopZ]], for the transfer of the Cu(I) cluster {{PubMed|19751213}}
| |
|
| |
| * '''[[Localization]]:''' cell membrane (according to Swiss-Prot)
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2RML 2RML] ( N-terminal soluble domain)
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/O32220 O32220]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33500]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' ''[[copZ]]-[[copA]]'' {{PubMed|12779235}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=copA_3441121_3443529_-1 copA] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:''' [[SigA]] {{PubMed|12779235}}
| |
| * '''Regulation:'''
| |
| ** induced by copper ([[CsoR]]) {{PubMed|18048925,12779235}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[CsoR]]: transcription repression {{PubMed|18048925}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
| [[John Helmann]], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed>19824702 23046649</pubmed>
| |
|
| |
| ==Original publications==
| |
| '''Additional publications:''' {{PubMed|22531974,22077885}}
| |
| <pubmed>19751213 11922674,12590580,14663075,12779235,12644235,18048925,14514665,14711369, 19378562 15212800 20233928 </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
