|
|
| (11 intermediate revisions by 4 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' phosphoenolpyruvate carboxykinase <br/><br/>
| | {{#bank: PckA}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''pckA''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ppc''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphoenolpyruvate carboxykinase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of phosphoenolpyruvate
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU30560 pckA]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/PckA PckA]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 58,1 kDa, 5.12
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1581 bp, 527 amino acids
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metK]]'', ''[[ytmB]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15034]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |-
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:pckA_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pckA_3129530_3131113_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pckA_expression.png|500px]]
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
| <br/><br/><br/><br/>
| |
|
| |
|
| |
| <br/><br/><br/><br/><br/><br/>
| |
|
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[carbon core metabolism]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CcpN regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU30560
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pckA.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11841]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO<sub>2</sub> (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
| |
|
| |
| * '''Protein family:''' phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
| ** Nucleotide binding Domain (233–240)
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[Mdh]]-[[PckA]] (this interaction occurs under gluconeogenic, but not under glycolytic conditions) {{PubMed|20933603}}
| |
|
| |
| * '''[[Localization]]:''' cytoplasm (according to Swiss-Prot), cytoplasm
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2PXZ 2PXZ] (''E.coli'')
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54418 P54418]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30560]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.1.49 4.1.1.49]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =Expression and regulation=
| |
| * '''Operon:''' ''[[pckA]]'' {{PubMed|9387221}}
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pckA_3129530_3131113_1 pckA] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:''' [[SigA]] {{PubMed|15720552}}
| |
|
| |
| * '''Regulation:'''
| |
| ** repressed by glucose (35-fold) [[CcpN]] {{PubMed|15720552,12850135}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[CcpN]]: transcription repression {{PubMed|15720552}}
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
| ** 1A1005 ( ''pckA''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1005&Search=1A1005 BGSC]
| |
| ** 1A996 ( ''pckA''::''spec''), {{PubMed| }}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A996&Search=1A996 BGSC]
| |
|
| |
| * '''Expression vector:'''
| |
| ** pGP1753 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
| |
| ** pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Stülke]] lab)
| |
| ** pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Stülke]] lab)
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| * '''FLAG-tag construct:''' GP1102 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| [[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
|
| |
| <pubmed>12850135 15720552 18586936, 9387221 20933603 22190493 </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
