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YfmL: Difference between revisions

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{{#bank: YfmL}}


{| align="right" border="1" cellpadding="2"
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|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''yfmL''
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
|-
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
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|style="background:#ABCDEF;" align="center"| '''Product''' || [[DEAD-box RNA helicases|DEAD-box RNA helicase]]
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|style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase
|-
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU07430 yfmL]
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.602 
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1128 bp, 376 aa
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfmM]]'', ''[[yfmK]]''
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12572&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:yfmL_context.gif]]
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfmL_816113_817243_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:yfmL_expression.png|500px]]
|-
|}
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= [[Categories]] containing this gene/protein =
{{SubtiWiki category|[[DEAD-box RNA helicases]]}}
= This gene is a member of the following [[regulons]] =
=The gene=
=== Basic information ===
* '''Locus tag:''' BSU07430
===Phenotypes of a mutant ===
=== Database entries ===
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfmL.html]
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12963]
=== Additional information===
=The protein=
=== Basic information/ Evolution ===
* '''Catalyzed reaction/ biological activity:''' RNA helicase
* '''Protein family:''' helicase C-terminal domain (according to Swiss-Prot) [[DEAD-box RNA helicases|DEAD-box RNA helicase]]
* '''Paralogous protein(s):''' [[CshA]], [[CshB]], [[DeaD]]
=== Extended information on the protein ===
* '''Kinetic information:'''
* '''Domains:'''
* '''Modification:'''
* '''Cofactor(s):'''
* '''Effectors of protein activity:'''
* '''[[SubtInteract|Interactions]]:'''
* '''[[Localization]]:'''
=== Database entries ===
* '''Structure:'''
* '''UniProt:''' [http://www.uniprot.org/uniprot/O34750 O34750]
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU07430]
* '''E.C. number:'''
=== Additional information===
=Expression and regulation=
* '''Operon:''' ''yfmL'' (according to [http://dbtbs.hgc.jp/COG/prom/yfmL.html DBTBS])
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfmL_816113_817243_1 yfmL] {{PubMed|22383849}}
* '''Sigma factor:'''
* '''Regulation:'''
* '''Regulatory mechanism:'''
* '''Additional information:'''
=Biological materials =
* '''Mutant:''' GP1053 (erm), available in the [[Stülke]] lab
* '''Expression vector:'''
       
* '''lacZ fusion:'''
* '''GFP fusion:''' B. subtilis GP1082 yfmL-yfp spc, available in [[Stülke]] lab,
* '''two-hybrid system:'''  B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
* '''FLAG-tag construct:''' GP1066 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
* '''Antibody:'''
=Labs working on this gene/protein=
=Your additional remarks=
=References=
[[Category:Protein-coding genes]]

Latest revision as of 10:11, 17 April 2014