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| * '''Description:''' transcriptional repressor of ''[[citZ]]'' and ''[[citB]]'' <br/><br/>
| | {{#bank: CcpC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ccpC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykuM ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional repressor ([[LysR family]])
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || regulation of tricarboxylic acid branch of the TCA cycle
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU14140 ccpC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 33 kDa, 5.932
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 879 bp, 293 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykuL]]'', ''[[ykuN]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13287]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ccpC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ccpC_1486045_1486926_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:ccpC_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[regulators of core metabolism]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CcpA regulon]]}},
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| {{SubtiWiki regulon|[[CcpC regulon]]}}
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| =The CcpC regulon:=
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| * ''[[ccpC]]'', ''[[citB]]'', ''[[citZ]]-[[icd]]-[[mdh]]''
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU14140
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ykuJK-ykzF-ykuL-ccpC.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13297]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' [[LysR family]]
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34827 O34827]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU14140]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[ykuJ]]-[[ykuK]]-[[abbA]]-[[ykuL]]-[[ccpC]]'' {{PubMed|16395550}}
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| ** ''[[ccpC]]'' {{PubMed|16395550}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ccpC_1486045_1486926_1 ccpC] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| ** ''[[ccpC]]'': [[SigA]]
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| * '''Regulation:'''
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| ** repressed in the presence of glucose and glutamate ([[CcpC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11985717 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[CcpC]]: transcription repression (molecular inducer: citrate) [http://www.ncbi.nlm.nih.gov/sites/entrez/11985717 PubMed]
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| ** [[CcpA]]: transcription repression {{PubMed|16395550}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP206 (''aphA3''), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| ** for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]]: pGP706, available in [[Stülke]] lab
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| * '''lacZ fusion:''' pGP707 (in [[pAC5]]), available in [[Stülke]] lab, pGP711 (in [[pAC6]]), available in [[Stülke]] lab
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Linc Sonenshein|Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>17994626,,11985717,10656796 12591885 14636591, 16395550</pubmed>
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| [[Category:Protein-coding genes]]
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