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| * '''Description:''' ATP-dependent deoxyribonuclease (subunit B) <br/><br/>
| | {{#bank: AddB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''addB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ATP-dependent deoxyribonuclease (subunit B))
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/AddB AddB]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 134 kDa, 5.39
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 3498 bp, 1166 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhjR]]'', ''[[addA]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12902]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:addB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=addB_1136320_1139820_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:addB_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DNA repair/ recombination]]}},
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| {{SubtiWiki category|[[genetic competence]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[ComK regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU10620
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/addBA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10465]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** the enzyme is functional as a heterodimer of the [[AddA]] and [[AddB]] subunits, that it is a rapid and processive DNA helicase, and that it catalyses DNA unwinding using one single-stranded DNA motor of 3'→5' polarity located in the [[AddA]] subunit {{PubMed|21071401}}
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| ** the [[AddB]] subunit contains a second putative ATP-binding pocket, but this does not contribute to the observed helicase activity and may instead be involved in the recognition of recombination hotspot sequences {{PubMed|21071401}}
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| * '''Protein family:''' uvrD-like helicase C-terminal domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[AddA]]-[[AddB]] {{PubMed|10756102,21071401}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=3U4Q 3U4Q] (the [[AddA]]-[[AddB]]-DNA complex) {{PubMed|22307084}}
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| ** [http://www.rcsb.org/pdb/explore.do?structureId=1Y26 1Y26] (the [[riboswitch]] in complex with adenine)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P23477 P23477]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10620]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[addB]]-[[addA]]'' {{PubMed|1646786}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=addB_1136320_1139820_1 addB] {{PubMed|22383849}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|7746142}}
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| * '''Regulation:'''
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| ** positive control by [[ComK]] {{PubMed|7746142}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP1106 (addAB, spc), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Mark Dillingham]], Bristol, U.K. ([http://www.bris.ac.uk/biochemistry/research/md.html homepage])
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>, 20116346 </pubmed>
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| ==Original publications==
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| '''Additional publications:''' {{PubMed|21071401}}
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| <pubmed>21809208,8387145,15610857,7746142, 19129187 1646786 10756102 9781875 17570399 20350930 22307084</pubmed>
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| [[Category:Protein-coding genes]]
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