|
|
| (16 intermediate revisions by 5 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' mediator of [[RecA]] binding to ssDNA <br/><br/>
| | {{#bank: RecO}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''recO''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqxN, yqfI ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || mediator of [[RecA]] binding to ssDNA
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || [[DNA repair/ recombination]]
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/RecO RecO]
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 8.208
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glyQ]]'', ''[[era]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14457]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |-
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:recO_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
|
| |
| <br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[DNA repair/ recombination]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
|
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU25280
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' no entry
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11189]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
| ** provides [[RecA]] access to ssDNA during chromosomal transformation (together with [[DprA]]) {{PubMed|22373918}}
| |
| ** catalyzes annealing of [[SsbA]] or [[SsbA]]/[[SsbB]] coated ssDNAs to allow the formation of DNA duplexes with tails during plasmid transformation {{PubMed|22373918}}
| |
|
| |
| * '''Protein family:''' recO family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''[[SubtInteract|Interactions]]:'''
| |
| ** [[SsbA]] (bound to ssDNA) -[[RecO]], this helps to dislodge [[SsbA]] and [[SsbB]] from ssDNA {{PubMed|21170359,22373918}}
| |
| ** [[RecR]]-[[RecO]], [[RecR]]:[[RecO]] molecular ratio of 2:1 (as judged from other bacteria such as ''E. coli'' and ''Deinococcus radiodurans'') {{PubMed|17581636}}
| |
|
| |
| * '''[[Localization]]:'''
| |
| ** cytoplasm (according to Swiss-Prot)
| |
| ** localizes to the DNA entry pole during transformation {{PubMed|22373918}}
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
| ** [http://www.rcsb.org/pdb/explore/explore.do?structureId=2V1C 2V1C] (the [[RecR]]-[[RecO]] complex from ''Deinococcus radiodurans'', [[RecR]]: 52% identity, 78% similarity, [[RecO]]: 30%/ 58%) {{PubMed|17581636}}
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P42095 P42095]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25280]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
|
| |
| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=recO_2608946_2609713_-1 recO] {{PubMed|22383849}}
| |
|
| |
| * '''Sigma factor:'''
| |
|
| |
| * '''Regulation:'''
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| '''Additional publications:''' {{PubMed|21170359}}
| |
| <pubmed>,18599486, 19730681 20581116 22373918 17581636</pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
