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| * '''Description:''' glucose 6-phosphate dehydrogenase, pentose-phosphate pathway <br/><br/>
| | {{#bank: Zwf}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''zwf''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqjJ ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || glucose 6-phosphate dehydrogenase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || initiation of the pentose phosphate pathway
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 55 kDa, 5.28
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1467 bp, 489 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rnz]]'', ''[[gndA]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14317]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:zwf_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU23850
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/zwf.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11739]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' D-glucose 6-phosphate + NADP<sup>+</sup> = D-glucono-1,5-lactone 6-phosphate + NADPH (according to Swiss-Prot)
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| * '''Protein family:''' glucose-6-phosphate dehydrogenase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Two Substrate Michaelis-Menten [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed]
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):''' Mg2+, Mn2+, Ca2+
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| * '''Effectors of protein activity:'''
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| ** NAD+, NADP+ and NADPH affect the enzyme kinetic [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed]
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.pdb.org/pdb/explore/explore.do?structureId=1DPG 1DPG] (from ''Leuconostoc Mesenteroides'', 45% identity, 62% similarity) {{PubMed|7881907}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54547 P54547]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23850]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.1.1.49 1.1.1.49]
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| === Additional information===
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| The enzyme is a dimer [http://www.ncbi.nlm.nih.gov/pubmed/6292660 PubMed]
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| =Expression and regulation=
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| * '''Operon:''' ''zwf'' [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' constitutive [http://www.ncbi.nlm.nih.gov/sites/entrez/12850135 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** SM-NB3 (''zwf-spc''), available in [[Anne Galinier]]'s and [[Boris Görke]]'s labs
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| * '''FLAG-tag construct:''' GP1401 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>6292660,12850135,19779711,7881907 </pubmed>
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| [[Category:Protein-coding genes]]
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