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| * '''Description:''' beta-ketoacyl-acyl carrier protein synthase II, involved in the control of membrane fluidity <br/><br/>
| | {{#bank: FabF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''fabF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yjaY ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |style="background:#ABCDEF;" align="center"| '''Product''' || beta-ketoacyl-acyl carrier protein synthase II
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| |style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid biosynthesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_synthesis.html Lipid synthesis]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 4.768
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1239 bp, 413 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fabHA]]'', ''[[yjaZ]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12975]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:fabF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[membrane dynamics]]}},
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| {{SubtiWiki category|[[biosynthesis of lipids]]}},
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| {{SubtiWiki category|[[cell envelope stress proteins (controlled by SigM, V, W, X, Y)]]}},
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| {{SubtiWiki category|[[essential genes]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[FapR regulon]]}},
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| {{SubtiWiki regulon|[[SigW regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU11340
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fabHAF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13128]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl-carrier-protein] + CO<sub>2</sub> + [acyl-carrier-protein] (according to Swiss-Prot)
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| ** [[SigW]]-dependent expression of ''[[fabF]]'' and the ''[[yuaF]]-[[floT]]-[[yuaI]]'' operon result in reduced membrane fluidity {{PubMed|21542858,22178969}}
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| * '''Protein family:''' beta-ketoacyl-ACP synthases family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=1OX0 1OX0] (the protein from ''Streptococcus pneumoniae'') {{PubMed|12837788}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34340 O34340]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU11340]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.179 2.3.1.179]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[fabHA]]-[[fabF]]'' {{PubMed|12737802}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[fabHA]]'': [[SigA]] {{PubMed|12737802}}
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| ** ''[[fabF]]'': [[SigW]] {{PubMed|21542858}}
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| * '''Regulation:'''
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| ** ''[[fabHA]]'': expressed when the cells experience a lack of malonyl-CoA ([[FapR]]) {{PubMed|12737802}}
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| ** ''[[fabHA]]'': induced upon fatty acid biosynthesis inhibition {{PubMed|21383089}}
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| ** ''[[fabF]]'': induced by alkaline shock and by polymyxin B, vancomycin, cephalosporin C, D-cycloserine, and triton X-100 ([[SigW]]) {{PubMed|21542858}}
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| * '''Regulatory mechanism:'''
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| ** [[FapR]]: transcription repression {{PubMed|12737802}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 15952903 17919287</pubmed>
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| ==Original Publications==
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| '''Additional publications:''' {{PubMed|21542858,22178969}}
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| <pubmed>18384517, 12837788,12737802, 12682299 21383089</pubmed>
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| [[Category:Protein-coding genes]]
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