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| * '''Description:''' indole-3-glycerol-phosphate synthase <br/><br/>
| | {{#bank: TrpC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''trpC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || indole-3-glycerol-phosphate synthase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of tryptophan
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phenyl_tyr_tryp.html Phe, Tyr, Trp]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 27 kDa, 5.535
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 750 bp, 250 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trpF]]'', ''[[trpD]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14182]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:trpC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[TRAP regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU22660
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| [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=trpC_2374139_2374888_-1 Expression]
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/trpEDCFBA-hisC-tyrA-aroE.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11038]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO<sub>2</sub> + H<sub>2</sub>O (according to Swiss-Prot)
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1VC4 1VC4] (from ''Thermus thermophilus'', 36% identity, 49% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P03964 P03964]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU22660]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.1.48 4.1.1.48]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[aroF]]-[[aroB]]-[[aroH]]-[[trpE]]-[[trpD]]-[[trpC]]-[[trpF]]-[[trpB]]-[[trpA]]-[[hisC]]-[[tyrA]]-[[aroE]]'' (according to [http://dbtbs.hgc.jp/COG/prom/trpEDCFBA-hisC-tyrA-aroE.html DBTBS])
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| ** ''[[trpE]]-[[trpD]]-[[trpC]]-[[trpF]]-[[trpB]]-[[trpA]]-[[hisC]]-[[tyrA]]-[[aroE]]'' {{PubMed|3924737}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[trpE]]'': [[SigA]] {{PubMed|6436812}}
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| * '''Regulation:'''
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| ** induction by tryptophan ([[MtrB|TRAP]]) {{PubMed|1551827}}
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| * '''Regulatory mechanism:'''
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| ** [[MtrB|TRAP]]: binding to the mRNA in the presence of tryptophan, this results in transcription termination {{PubMed|8419914}}
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| * '''Additional information:'''
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| ** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J'' </big>
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| <big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big>
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| <big>Mol Microbiol. 2011 81(6): 1459-1473. </big>
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| [http://www.ncbi.nlm.nih.gov/pubmed/21815947 PubMed:21815947]
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| <pubmed> 14976255, 3924737, 6436812,2422155,8419914, 1551827 </pubmed>
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| [[Category:Protein-coding genes]]
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