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| * '''Description:''' [[RNase]] EndoA, MazF family toxin, UACAU-specific mRNA interferase <br/><br/>
| | {{#bank: NdoA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ndoA''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ydcE''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || mRNA interferase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || unknown
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/NdoA NdoA]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 12 kDa, 4.892
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 348 bp, 116 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ndoAI]]'', ''[[rsbR]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12273]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ydcE_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[Rnases]]}},
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| {{SubtiWiki category|[[toxins, antitoxins and immunity against toxins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU04660
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| [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=ndoA_518943_519293_1 Expression]
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ydcE.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12092]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' specifically cleaves a five-base sequence, UACAU {{PubMed|21763692}}
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| * '''Protein family:''' pemK family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' the activity is inhibited by the interaction with [[NdoAI]] {{PubMed|17416361}}
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[NdoAI]]-[[NdoA]] {{PubMed|17416361}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1NE8 1NE8]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P96622 P96622]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04660]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ndoAI]]-[[ndoA]]'' {{PubMed|15882409}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| ** An [[ncRNA|antisense RNA]] is predicted for ''[[ndoA]]'' {{PubMed|20525796}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>15882409,17416361, 20525796 21763692 21897862 </pubmed>
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| [[Category:Protein-coding genes]]
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