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| * '''Description:''' MarR/DUF24-family transcription regulator, positively controls the nitroreductase gene ''[[yfkO]]'' (hypO) in response to disulfide stress <br/><br/>
| | {{#bank: HypR}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''yybR''(hypR)
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''hypR''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || MarR/DUF24-family transcription regulator HypR
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| |style="background:#ABCDEF;" align="center"|'''Function''' || positively controls nitroreductase gene hypO (yfkO) in response to disulfide stress (diamide, NaOCl)
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 14 kDa, 8.415
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 375 bp, 125 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cotF]]'', ''[[ppaC]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB16091]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yybR_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU40540
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| ===Phenotypes of a mutant ===
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| ''' hypO ohrA double mutant more sensitive to NaOCl stress than ohrA single mutant
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yybR.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10013]
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| === Additional information===
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| ''' hypR autoregulated by disulfide stress
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' MarR/DUF24-family regulator
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| * '''Paralogous protein(s):''' YdeP,YkvN
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Cys14 redox sensing Cys, has lower pKa of 6.36
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| * '''Domains:''' 5 alpha helices, 2 beta sheets, MarR-fold with wHTH motif, alpha4 major groove recognition helix, beta2 and 3 form the wing; alpha5 dimer interface
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| * '''Modification:''' oxidized to Cys14-Cys49' intersubunit disulfides by disulfide stress
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:''' cytoplasmic
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| === Database entries ===
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| * '''Structure:''' tba
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P37486 P37486]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40540]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''yybR'' (hypR)(according to [http://dbtbs.hgc.jp/COG/prom/yybR.html DBTBS])
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' activated by disulfide stress conditions (diamide, NaOCl) in vivo and in vitro
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| * '''Regulatory mechanism:''' redox-controlled by Cys14-Cys49' intersubunit disulfide formation by diamide and NaOCl in vitro and vivo
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| * '''Additional information:''' Cys14 and Cys49' are about 8-9 Angstroem apart in reduced HypR-Dimer, oxidation moves the major groove recognition alpha helices of the HypR dimer about 4 Angstroem towards each other
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Haike Antelmann]],University of Greifswald, Germany
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| =Your additional remarks=
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| =References=
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| '''Palm, Gottfried; Chi, Bui Khanh; Waack, Paul; Gronau, Katrin; Becher, Dörte; Albrecht, Dirk; Hinrichs, Winfried; Read, Randy and Antelmann, Haike
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| Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR
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| Nucleic Acid Research 2012, in press.
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| [[Category:Protein-coding genes]]
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