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| * '''Description:''' antagonist of [[SinR]], drives [[SlrR]] from the [[SlrR]](LOW) to the [[SlrR]](HIGH) state <br/><br/>
| | {{#bank: SinI}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sinI''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || antagonist of [[SinR]]
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[biofilm formation]]
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SinI SinI]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/biofilm.html Biofilm]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 6.333
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 171 bp, 57 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqhG]]'', ''[[sinR]]''
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| |-
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14391]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sinI_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[transcription factors and their control]]}},
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| {{SubtiWiki category|[[transition state regulators]]}},
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| {{SubtiWiki category|[[biofilm formation]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[AbrB regulon]]}},
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| {{SubtiWiki regulon|[[ScoC regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24600
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sinIR.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10753]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[SlrA]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SinR]]-[[SinI]] {{PubMed|9799632}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1B0N 1B0N] (complex [[SinR]]-[[SinI]]) {{PubMed|9799632}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P23308 P23308]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24600]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' [[sinI]]-[[sinR]] {{PubMed|3125149}}, [[sinI]]-[[Spo0A]] {{PubMed|21326214}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|3125149}}
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| * '''Regulation:'''
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| ** activated under conditions that trigger sporulation by ([[Spo0A]]) interaction [http://www.ncbi.nlm.nih.gov/sites/entrez/11751836 PubMed],{{PubMed|21326214}}
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| ** repressed during exponential growth ([[ScoC]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
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| ** repressed during logrithmic growth ([[AbrB]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
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| ** repressed during vegetative growth ([[SinR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1664536 PubMed]
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription activation, in a portion of cells [http://www.ncbi.nlm.nih.gov/sites/entrez/11751836 PubMed]
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| ** [[ScoC]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
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| ** [[AbrB]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
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| ** [[SinR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1664536 PubMed]
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| * '''Additional information:'''
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| ** the mRNA is substantially stabilized upon depletion of [[Rny|RNase Y]] {{PubMed|21815947}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** GP959 (spc), available in [[Stülke]] lab
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| ** DS91 (spc) NCIB3610 derivate, available in [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''FLAG-tag construct (C-term):''' GP935 (kan), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/ protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20541494 </pubmed>
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| ==Modelling of the [[SinI]]/[[SinR]] switch==
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| <pubmed> 21095906 </pubmed>
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| ==Original publications==
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| '''Additonal publications:''' {{PubMed|21326214}}
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| <big>''Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J'' </big>
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| <big>'''RNA processing in ''Bacillus subtilis'': identification of targets of the essential RNase Y.''' </big>
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| <big>Mol Microbiol. 2011 81(6): 1459-1473. </big>
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| [http://www.ncbi.nlm.nih.gov/pubmed/21815947 PubMed:21815947]
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| <pubmed>3125149 15661000,18047568,,11751836,1906467,11751836,7635837,11751836, 10547280, 15104138, 9799632 20923420 8422983 </pubmed>
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| [[Category:Protein-coding genes]]
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