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| * '''Description:''' succinate dehydrogenase (cytochrome b558 subunit) <br/><br/>
| | {{#bank: SdhC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sdhC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || succinate dehydrogenase (cytochrome b558 subunit)
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://cellpublisher.gobics.de/subtinteract/startpage/start/ ''Subt''Interact]''': [http://cellpublisher.gobics.de/subtinteract/interactionList/2/SdhC SdhC]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 22 kDa, 9.831
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 606 bp, 202 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[sdhA]], [[yslB]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14805]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sdhC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[membrane proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[FsrA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU28450
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sdhCAB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10351]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:''' cytochrome b558 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):''' Fe
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| * '''Effectors of protein activity:'''
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| ** Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed]
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| ** Activated by Cytochrome b558 [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[SdhB]]-[[SdhC]] (as part of the [[SdhC]]-[[SdhB]]-[[SdhA]] complex)
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| * '''[[Localization]]:''' membrane protein [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NEK 1NEK] (''E. coli'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P08064 P08064]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28450]
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| * '''E.C. number:''' 1.3.99.1
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| === Additional information===
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| * This enzyme is a trimer membrane-bound [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
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| ** One subunit is bound to citochrome b558, and this subunit is the one bound to the cytosolic side of the membrane [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
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| ** Another subunit is the flavoprotein one, required for FAD usage [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
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| ** The other subunit has an iron-sulphur domain necessary for the catalytic activity [http://www.ncbi.nlm.nih.gov/pubmed/3910107 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/6799760 PubMed]
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| * extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at [http://www.proteopedia.org/wiki/index.php/Succinate_Dehydrogenase Proteopedia]
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| =Expression and regulation=
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| * '''Operon:''' ''[[sdhC]]''-''[[sdhA]]''-''[[sdhB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/3036777 PubMed]
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| * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2495271 PubMed]
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| * '''Regulation:''' SdhC is less expressed under conditions of extreme iron starvation ([[FsrA]]) [http://www.ncbi.nlm.nih.gov/pubmed/18697947 PubMed]
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| * '''Regulatory mechanism:''' [[FsrA]]: translational repression, [[sRNA]] [http://www.ncbi.nlm.nih.gov/pubmed/18697947 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP743 (''sdhCA'', cat), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>6401289,1707123,2120540,7669765,18697947,1324713,3036777,2495271,2176107,3117551,10913269,18763711,12560550, 18697947 3910107 6799760 21641999 </pubmed>
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| [[Category:Protein-coding genes]]
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