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| * '''Description:''' ATP-dependent Clp protease proteolytic subunit (class III heat-shock protein) <br/><br/>
| | {{#bank: ClpP}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''clpP''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yvdN ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || ATP-dependent Clp protease proteolytic subunit
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Function''' || protein degradation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phosphorelay.html Phosphorelay], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 5.008
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 591 bp, 197 aa
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| |-
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trnQ-Arg]]'', ''[[pgcM]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15459]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:clpP_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[proteolysis]]}},
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| {{SubtiWiki category|[[general stress proteins (controlled by SigB)]]}},
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| {{SubtiWiki category|[[heat shock proteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CtsR regulon]]}},
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| {{SubtiWiki regulon|[[SigB regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU34540
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/clpP.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG19016]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Hydrolysis of proteins to small peptides in the presence of ATP and magnesium (according to Swiss-Prot) endopeptidase/proteolysis
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| * '''Protein family:''' peptidase S14 family (according to Swiss-Prot) ClpP (IPR001907) [http://www.ebi.ac.uk/interpro/DisplayIproEntry?ac=IPR001907 InterPro], (PF00574) [http://pfam.sanger.ac.uk/family?acc=PF00574 PFAM]
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| * '''Paralogous protein(s):'''
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| === Targets of [[ClpC]]-[[ClpP]]-dependent protein degradation ===
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| * [[DegU]]-P {{PubMed|20070525}}
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| ** [[ClpE]]-[[ClpP]], [[ClpC]]-[[ClpP]], [[ClpX]]-[[ClpP]]
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| ** [[CtsR]]-[[ClpP]]/[[ClpC]] (degradation of [[CtsR]]) {{PubMed|11179229,16163393,17380125}}
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| ** [[McsB]]-[[ClpP]]/[[ClpC]] (rapid degradation of non-phosphorylated [[McsB]]) {{PubMed|20852588}}
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| ** [[Spx]]-[[ClpP]]/[[ClpX]] (degradation of [[Spx]]) {{PubMed|17827297}}
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| * '''Localization:''' cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [[ClpX]], [[ClpC]] and [[ClpE]] {{PubMed|18786145,18689473}}
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| [[File:ClpP.jpg ]]
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| === Database entries ===
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| * '''Structure:''' [http://www.pdb.org/pdb/search/structidSearch.do?structureId=3KTG 3KTG] {{PubMed|20305655}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P80244 P80244]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34540]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.4.21.92 3.4.21.92]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''clpP'' {{PubMed|9643546}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9643546}}, [[SigB]] {{PubMed|9643546,11544224}}
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| * '''Regulation:'''
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| ** induced by stress ([[SigB]]) {{PubMed|9643546,11544224}}
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| ** induced by heat ([[CtsR]]) {{PubMed|9987115}}
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| * '''Regulatory mechanism:'''
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| ** [[CtsR]]: transcription repression {{PubMed|9987115,11179229,16163393,17380125}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| ** ''clpP::spec'' and ''clpP::cat'', available in the [[Leendert Hamoen]] lab
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| ** GP551 (spc), available in the [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:''' C-terminal GFP fusions (both single copy and as 2th copy in ''amyE'' locus, also as CFP and YFP variants) available in the [[Leendert Hamoen]] lab
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Leendert Hamoen]], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| {{PubMed|19609260,19781636}}
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| ==Original Publications==
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| <pubmed>9643546,10809708,11807061,9987115,14679237,18689476,15317791,17586624,11684022,12923101,17560370, 16899079,19226326 , 20070525,9987115,11544224 14763982 9643546, 19767395 11112444 9535081 18689473 20305655 20852588 </pubmed>
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| Additional publication: {{PubMed|17380125,12598648,9890793,20049702,20049702}}
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| [[Category:Protein-coding genes]]
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