|
|
| (13 intermediate revisions by 2 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' long chain acyl-CoA synthetase, involved in surfactin production <br/><br/>
| | {{#bank: LcfA}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''lcfA''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || long chain acyl-CoA synthetase
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || fatty acid degradation
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/fatty_acid_deg.html Fatty acid degradation]'''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62 kDa, 6.119
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1680 bp, 560 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fadR]]'', ''[[yshE]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14816]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:lcfA_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
|
| |
| <br/><br/><br/><br/><br/><br/>
| |
|
| |
| = [[Categories]] containing this gene/protein =
| |
| {{SubtiWiki category|[[utilization of lipids]]}}
| |
|
| |
| = This gene is a member of the following [[regulons]] =
| |
| {{SubtiWiki regulon|[[CcpA regulon]]}},
| |
| {{SubtiWiki regulon|[[FadR regulon]]}}
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU28560
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lcfA-ysiAB-etfBA.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11946]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA (according to Swiss-Prot), activates 3-hydroxy fatty acids for surfactin biosynthesis {{PubMed|20797616}}
| |
|
| |
| * '''Protein family:''' ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):''' [[LcfB]], [[YhfT]], [[YngI]]
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''Interactions:'''
| |
|
| |
| * '''Localization:''' cytoplasm (according to Swiss-Prot)
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P94547 P94547]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28560]
| |
|
| |
| * '''E.C. number:''' [http://www.expasy.org/enzyme/6.2.1.3 6.2.1.3]
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''[[lcfA]]-[[fadR]]-[[fadB]]-[[etfB]]-[[etfA]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
|
| |
| * '''Regulation:'''
| |
| ** subject to carbon catabolite repression ([[CcpA]]-[[PtsH|HPr(Ser-P)]] {{PubMed|21398533}}
| |
| ** induced by long chain acyl-CoA (C14 ... C20) ([[FadR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** [[CcpA]]-[[PtsH|HPr(Ser-P)]]: transcription repression {{PubMed|21398533}}
| |
| ** [[FadR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/17189250 PubMed]
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:''' available in [[Mohamed Marahiel]]'s lab {{PubMed|20797616}}
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| '''Additional publications:''' {{PubMed|21398533}}
| |
| <pubmed>17189250,17919287 20797616 17085570 </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
