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| * '''Description:''' biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) <br/><br/>
| | {{#bank: TsaC}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ywlC''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ipc-29d ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || unknown
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| |style="background:#ABCDEF;" align="center"|'''Function''' || tRNA modification
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 36 kDa, 5.82
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1038 bp, 346 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ywlD]]'', ''[[ywlB]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15712]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ywlC_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[translation]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[DnaA regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU36950
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10939]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding ''E. coli'' protein YrdC) {{PubMed|19287007}}
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| * '''Protein family:''' SUA5 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1HRU 1HRU] (the homolog from ''E. coli'') {{PubMed|19287007}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39153 P39153]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU36950]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| ** [[DnaA]]: transcription repression {{PubMed|17932079}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>17005971 17932079 19287007 </pubmed>
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| [[Category:Protein-coding genes]]
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