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| * '''Description:''' write here <br/><br/>
| | {{#bank: Obg}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''obg''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || Yes
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| |style="background:#ABCDEF;" align="center"| '''Product''' || GTPase activity
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| |style="background:#ABCDEF;" align="center"|'''Function''' || may be required to stimulate activity of the phosphorelay that activates Spo0A
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 47 kDa, 4.859
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1284 bp, 428 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || spo0B, pheB
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/obg_nucleotide.txt Gene sequence (+200bp) ]'''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/obg_protein.txt Protein sequence]'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:obg_context.gif]]
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:'''
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/obg.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10337]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| Binds and hydrolyzes GTP and readily exchanges GDP for GTP
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| N-terminal glycine-rich domain (1–158), GTP binding domain (159–342), C-terminal TGS domain (343–428). The TGS domain has no known function, but it is named for three protein families in which it is found, ThrRS, GTPase, and SpoT.
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| Interaction with Rsb proteins.
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=1LNZ]
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| * '''Swiss prot entry:'''
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| * '''KEGG entry:'''
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| [http://www.genome.ad.jp/dbget-bin/www_bget?bsu+BSU27920]
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| * '''E.C. number:'''
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| === Additional information===
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| The biochemical analysis of bacterial and human Obg proteins combined with the structural observation of the ppGpp nucleotide within the Obg active site suggest a potential role for ppGpp modulation of Obg function in ''B. subtilis''.
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| =Expression and regulation=
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| * '''Operon:'''
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| [[spo0B]]-obg
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| * '''Sigma factor:'''
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| One SigA-dependent promoter upstream of [[spo0B]]
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| # Buglino J., Shen V., Hakiman P. & Lima C.D. (2002) Structural and biochemical analysis of the Obg GTP binding protein ''Structure'' '''10:''' 1581-1592. [http://www.ncbi.nlm.nih.gov/pubmed/12429099?ordinalpos=3&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
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| # Scott, J.M., Ju, J., Mitchell, T., and Haldenwang, W.G. (2000) The Bacillus subtilis GTP binding protein obg and regulators of the sigma(B) stress response transcription factor cofractionate with ribosomes ''J. Bacteriol.'' '''182:''' 2771–2777. [http://www.ncbi.nlm.nih.gov/pubmed/10781545?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum]
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