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| * '''Description:''' transcriptional activator of arginine utilization operons <br/><br/>
| | {{#bank: RocR}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rocR''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator
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| |style="background:#ABCDEF;" align="center"|'''Function''' || transcriptional activator of arginine utilization operons
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 52.6 kDa, 6.08
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1383 bp, 461 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rocD]]'', ''[[yyxA]]''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/rocR_nucleotide.txt Gene sequence (+200bp) ]'''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/rocR_protein.txt Protein sequence]'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rocR_context.gif]]
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:''' 4144771 - 4146153
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocR.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10723]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' transcription activation at [[SigL]]-dependent promoters of ''[[rocA|rocABC]]'', ''[[rocD|rocDEF]]'', and ''[[rocG]]''
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| * '''Protein family:''' [[SigL | Sigma-54]] interacting transcription activator
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| **[[SigL | Sigma-54]] factor interaction domain (143–372)
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| **2x nucleotid-binding domain (171–178),(233–242)
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| **HTH motif (434–453)
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| * '''Modification:'''
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| * '''Cofactor(s):''' ornithine or citrulline are required for RocR-dependent transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9194709 PubMed]
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' RocR-[[SigL]]
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.expasy.ch/cgi-bin/sprot-search-ac?P38022]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU40350]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''rocR''
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| * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+7540694 PubMed]
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| * '''Regulation:''' autoregulation by [[RocR]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+7540694 PubMed]
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| * '''Regulatory mechanism:''' autorepression
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' QB5533 (aphA3), from [[Michel Debarbouille]], available in [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Michel Debarbouille]], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
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| =Your additional remarks=
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| =References=
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| # Belitsky BR, Sonenshein AL (1998) Role and regulation of Bacillus subtilis glutamate dehydrogenase genes. J Bacteriol 180:6298-6305. [http://www.ncbi.nlm.nih.gov/sites/entrez/9829940 PubMed]
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| # Belitsky BR, Sonenshein, AL: (1999) An enhancer element located downstream of the major glutamate dehydrogenase gene of Bacillus subtilis. Proc Natl Acad Sci USA , 96:10290-10295. [http://www.ncbi.nlm.nih.gov/sites/entrez/10468601 PubMed]
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| # Gardan R, Rapoport G, Débarbouillé M:(1997) Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis. Mol Microbiol , 24:825-837. [http://www.ncbi.nlm.nih.gov/sites/entrez/9194709 PubMed]
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| # Gardan R, Rapoport G, Débarbouillé M: (1995) Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis. J Mol Biol , 249:843-856. [http://www.ncbi.nlm.nih.gov/sites/entrez/7540694 PubMed]
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| # Ali, N. O., J. Jeusset, E. Larquet, E. le Cam, B. Belitsky, A. L. Sonenshein, T. Msadek, and M. Débarbouillé. 2003. Specificity of the interaction of RocR with the ''rocG-rocA'' intergenic region in ''Bacillus subtilis''. Microbiology 149: 739-750. [http://www.ncbi.nlm.nih.gov/sites/entrez/12634342 PubMed]
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