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| * '''Description:''' phosphoenolpyruvate carboxykinase <br/><br/>
| | {{#bank: PckA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pckA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ppc''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphoenolpyruvate carboxykinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of phosphoenolpyruvate
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 58,1 kDa, 5.12
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1581 bp, 527 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[metK]]'', ''[[ytmB]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15034]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pckA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU30560
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pckA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11841]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO<sub>2</sub> (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
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| * '''Protein family:''' phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| ** Nucleotide binding Domain (233–240)
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| ** [[Mdh]]-[[PckA]] (this interaction occurs under gluconeogenic, but not under glycolytic conditions) {{PubMed|20933603}}
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| * '''Localization:''' cytoplasm (according to Swiss-Prot), cytoplasm
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2PXZ 2PXZ] (''E.coli'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54418 P54418]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU30560]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.1.49 4.1.1.49]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[pckA]]'' {{PubMed|9387221}}
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| * '''Sigma factor:''' [[SigA]] {{PubMed|15720552}}
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| * '''Regulation:'''
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| ** repressed by glucose (35-fold) [[CcpN]] {{PubMed|15720552,12850135}}
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| * '''Regulatory mechanism:'''
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| ** [[CcpN]]: transcription repression {{PubMed|15720552}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| ** pGP1753 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
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| ** pGP1762 (for expression, purification in ''E. coli'' with N-terminal His-tag, in [[pWH844]], available in [[Stülke]] lab)
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| ** pGP1763 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [[pGP172]], available in [[Stülke]] lab)
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| * '''FLAG-tag construct:''' GP1102 (spc, based on [[pGP1331]]), available in the [[Stülke]] lab
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| =Labs working on this gene/protein=
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| [[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
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| =Your additional remarks=
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| =References=
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| <pubmed>12850135 15720552 18586936, 9387221 20933603</pubmed>
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| [[Category:Protein-coding genes]]
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