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| * '''Description:''' [[trigger enzymes|trigger enzyme]]: catabolic glutamate dehydrogenase induced by arginine, ornithine or proline, subject to carbon catabolite repression <br/><br/>
| | {{#bank: RocG}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''rocG''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[trigger enzymes|trigger enzyme]]: glutamate dehydrogenase (major)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || arginine utilization, controls the activity of [[GltC]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/glutamate.html Ammonium/ glutamate]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 46.2 kDa, 6.28
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1272 bp, 424 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[rocA]]'', ''[[yweA]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15806]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:rocG_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37790
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| ===Phenotypes of a mutant ===
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| Poor growth on complex media such as SP (sporulation medium). No growth in minimal media with arginine as the only carbon source. Rapid accumulation of suppressor mutants ([[gudB |''gudB1'']])
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rocG.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10621]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' L-glutamate + H<sub>2</sub>O + NAD<sup>+</sup> = 2-oxoglutarate + NH<sub>3</sub> + NADH (according to Swiss-Prot) L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH, controls the activity of the [[GltC]] transcription activator [http://www.ncbi.nlm.nih.gov/sites/entrez/17608797 PubMed]
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| * '''Protein family:''' Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot) Glu/Leu/Phe/Val dehydrogenases family
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| * '''Paralogous protein(s):''' [[GudB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' RocG-[[GltC]], this interaction prevents transcription activation of the ''[[gltA]]-[[gltB]]'' operon by GltC [http://www.ncbi.nlm.nih.gov/sites/entrez/17608797 PubMed]
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/search/structidSearch.do?structureId=3K92 3K92] (super-repressor mutant that is capable of constitutive inactivation of [[GltC]], E93K mutation) {{PubMed|20630473}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39633 P39633]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37790]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/1.4.1.2 1.4.1.2]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''rocG'' {{PubMed|10468601}}
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| * '''Sigma factor:''' [[SigL]] {{PubMed|10468601}}
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| * '''Regulation:'''
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| ** induced by arginine ([[RocR]], [[AhrC]]), ornithine or proline {{PubMed|10468601,12634342,17183217}}
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| ** subject to carbon catabolite repression ([[CcpA]]) {{PubMed|15150224}}
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| * '''Regulatory mechanism:'''
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| ** [[RocR]]: transcription activation {{PubMed|10468601,12634342}}
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| ** [[AhrC]]: transcription activation {{PubMed|17183217}}
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| ** [[CcpA]]: transcription repression {{PubMed|15150224}}
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| ** [[AbrB]]: transcription repression {{PubMed|20817675}}
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| * '''Additional information:'''
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| Activation by [[RocR]] requires binding of [[RocR]] to a downstream element {{PubMed|12634342}}
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| =Biological materials =
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| * '''Mutant:''' GP747 (spc), GP726 (aphA3), GP810 (tet) available in [[Stülke]] lab
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| * '''Expression vector:'''
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| ** expression of native ''rocG'' in ''B. subtilis'': pGP529 (in [[pBQ200]]), available in [[Stülke]] lab
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| ** for purification of RocG carrying an N-terminal Strep-tag: pGP902 (in [[pGP172]]), a series of ''rocG'' variants is also available in [[pGP172]], available in [[Stülke]] lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:''' available in [[Stülke]] lab
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| =Labs working on this gene/protein=
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| [[Linc Sonenshein|Linc Sonenshein]], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
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| [[Stülke|Jörg Stülke]], University of Göttingen, Germany
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| [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| =Your additional remarks=
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| =References=
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| '''Enzymatic activity of RocG'''
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| <pubmed>18603778,,16244435 16195607 ,18326565, 9829940 20630473 </pubmed>
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| '''Function in the control of [[GltC]] activity'''
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| <pubmed>15150225,17994626 ,17608797 17183217 20630473 </pubmed>
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| '''Expression of ''rocG'''''
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| <pubmed>12634342,15150224 10468601 9829940 20817675 </pubmed>
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| '''Structural analysis of glutamate dehydrogenase'''
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| <pubmed>8263917 20630473 </pubmed>
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| [[Category:Protein-coding genes]]
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