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| * '''Description:''' phenylalanyl-tRNA synthetase (alpha subunit), [[universally conserved protein]] <br/><br/>
| | {{#bank: PheS}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pheS''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phenylalanyl-tRNA synthetase (alpha subunit)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || translation
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/tRNA_charging.html tRNA charging]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 38 kDa, 5.083
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1032 bp, 344 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pheT]]'', ''[[ysgA]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14824]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pheS_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU28640
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| ===Phenotypes of a mutant ===
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| essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pheST.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10874]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe) (according to Swiss-Prot)
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| * '''Protein family:''' Phe-tRNA synthetase alpha chain type 1 subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' [[PheS]]-[[PheT]]
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| * '''Localization:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1PYS 1PYS] (from ''Thermus thermophilus hb8 mutant'', 37% identity, 56% similarity) {{PubMed|7664121}}
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P17921 P17921]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU28640]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/6.1.1.20 6.1.1.20]
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| === Additional information===
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| [[universally conserved protein]]
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| =Expression and regulation=
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| * '''Operon:''' ''[[pheS]]-[[pheT]]'' {{PubMed|2127701}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|2127701}}
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| * '''Regulation:'''
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| ** induced by phenylalanine limitation ([[T-box]]) {{PubMed|19258532}}
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| * '''Regulatory mechanism:'''
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| ** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>19258532,2127701 , </pubmed>
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| [[Category:Protein-coding genes]]
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