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| * '''Description:''' phosphate butyryltransferase <br/><br/>
| | {{#bank: Ptb}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''ptb''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqiS, bkd ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || phosphate butyryltransferase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of branched-chain keto acids
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/ile_val_leu.html Ile, Leu, Val]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 7.936
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 897 bp, 299 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bcd]]'', ''[[bkdR]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14340]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:ptb_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24090
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ptb-bcd-buk-lpdV-bkdAABB.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11722]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Butanoyl-CoA + phosphate = CoA + butanoylphosphate (according to Swiss-Prot)
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| * '''Protein family:''' phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?pdbId=1YCO 1YCO] (from ''Enterococcus faecalis v583'', 36% identity, 56% similarity)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P54530 P54530]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24090]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.3.1.19 2.3.1.19]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]'' {{PubMed|12823818}}
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| * '''[[Sigma factor]]:''' [[SigL]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
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| * '''Regulation:'''
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| ** induced in the presence of isoleucine or valine ([[BkdR]]) {{PubMed|10094682}}
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| * '''Regulatory mechanism:'''
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| ** [[CodY]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10094682 PubMed]
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| ** [[BkdR]]: transcription activation {{PubMed|10094682}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>12427936,15241682,12823818,10094682, </pubmed>
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| [[Category:Protein-coding genes]]
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