|
|
| (21 intermediate revisions by 5 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' phospholipase implicated in spore germination <br/><br/>
| | {{#bank: LipC}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''lipC''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycsK''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || spore coat phospholipase B
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || spore germination
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 5.839
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 639 bp, 213 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[kipR]]'', ''[[yczI]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12218]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:ycsK_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
|
| |
| <br/><br/>
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU04110
| |
|
| |
| ===Phenotypes of a mutant ===
| |
| defective in L-alanine-stimulated germination {{PubMed|20057119}}
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ycsFGI-kipIAR-ycsK.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11232]
| |
|
| |
| === Additional information===
| |
|
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:''' cleaves the fatty acids at the sn-1 and sn-2 positions of phospholipids {{PubMed|20057119}}
| |
|
| |
| * '''Protein family:''' 'GDSL' lipolytic enzyme family (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''Interactions:'''
| |
|
| |
| * '''Localization:''' spore coat {{PubMed|17220230,20057119}}
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:'''
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P42969 P42969]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU04110]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:'''
| |
| ** ''[[ycsF]]-[[ycsG]]-[[ycsI]]-[[kipI]]-[[kipA]]-[[kipR]]-[[lipC]]'' {{PubMed|9334321}}
| |
| ** ''lipC'' (according to [http://dbtbs.hgc.jp/COG/prom/ycsFGI-kipIAR-ycsK.html DBTBS])
| |
|
| |
| * '''[[Sigma factor]]:'''
| |
| ** ''lipC'': [[SigK]] {{PubMed|15699190,17220230}}
| |
|
| |
| * '''Regulation:'''
| |
| ** ''lipC'': expressed four hours after onset of sporulation ([[GerE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/17220230 PubMed]
| |
|
| |
| * '''Regulatory mechanism:'''
| |
| ** ''[[ycsF]]'': [[KipR]]: transcription repression {{PubMed|9334321}}
| |
| ** ''[[ycsF]]'': [[TnrA]]: transcription activation {{PubMed|9334321}}
| |
| ** [[GerE]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/17220230 PubMed]
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:'''
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| <pubmed> 17220230 9334321 20057119 </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
