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| * '''Description:''' fumarase<br/><br/>
| | {{#bank: CitG}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''citG''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || fumarate hydratase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || TCA cycle
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 50 kDa, 5.475
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1386 bp, 462 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[gerAA]], [[yuxN]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15294]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:citG_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU33040
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/citG.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10384]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' (S)-malate = fumarate + H<sub>2</sub>O (according to Swiss-Prot)
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| * '''Protein family:''' Fumarase subfamily (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[AnsB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:''' cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1YFE 1YFE] (''E.coli'')
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P07343 P07343]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU33040]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.2.1.2 4.2.1.2]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[citG]]''
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| * '''Sigma factor:''' [[SigA]], [[SigH]]
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| * '''Regulation:''' constitutive
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP718 (spec), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| ** pGP1122 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
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| * '''lacZ fusion:''' pGP387 (in [[pAC7]]), available in [[Stülke]] lab
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>2509422,, </pubmed>
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| [[Category:Protein-coding genes]]
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