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| * '''Description:''' developmental checkpoint protein, inhibits the autokinase activity of [[KinA]]
| | {{#bank: Sda}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sda''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || developmental checkpoint protein
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| |style="background:#ABCDEF;" align="center"|'''Function''' || mediates a developmental checkpoint coupling<br/>initiation of sporulation (phosphorylation of [[Spo0A]])<br/>to the function of replication initiation proteins
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 6 kDa, 5.605
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 156 bp, 52 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yloV]]'', ''[[sdaAA]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAE01463]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sda_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU25690
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| ===Phenotypes of a mutant ===
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| *sporulates at a higher frequency
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| *will sporulate in the presence of replication stress
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sda.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG14183]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Sda is a checkpoint protein that is upregulated in response to replication stress {{PubMed|11207367}}. Sda inhibits the autokinase activity of [[KinA]] (and likely [[KinB]] as well). This in its turn results in reduced levels of [[Spo0A|Spo0A~P]]. Thus, replication stressed cells will not initiate sporulation<br/><br/> [[Sda]] is rapidly turned over by the [[ClpP|ClpP/X]] system because of some uncharged residues at its C-terminus {{PubMed|16796683}}.
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' [[KinB]]-[[Sda]], [[KinA]]-[[Sda]]
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=3D36 3D36] (from Geobacillus stearothermophilus, complex with [[KinB]]), [http://www.rcsb.org/pdb/explore.do?structureId=1PV0 1PV0]
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/Q7WY62 Q7WY62]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25690]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[sda]]''
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| * '''Sigma factor:''' [[SigA]]
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| * '''Regulation:''' [[DnaA]]
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| ** repressed when no DNA damage is present ([[LexA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed]
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| * '''Regulatory mechanism:''' Probably activated by [[DnaA]] under replication stress
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| ** [[LexA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16267290 PubMed]
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| Bill Burkholder [http://burkholder.stanford.edu/ Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>11207367, 16267290, 19465772, 15023339 16796683</pubmed>
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| [[Category:Protein-coding genes]]
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