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| * '''Description:''' Sigma subunit of the RNA polymerase, Sigma-54, Sigma L <br/><br/>
| | {{#bank: SigL}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''sigL''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sigma-54 factor (sigma-L)
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| |style="background:#ABCDEF;" align="center"|'''Function''' || utilization of arginin, acetoin and fructose, <br/>required for cold adaptation
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 49,5 kDa, 7.79
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1308 bp, 436 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yvfG]]'', ''[[yvfH]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15425]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:sigL_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU34200
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| ===Phenotypes of a mutant ===
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| The mutant is cold-sensitive and unable to use arginine as a single carbon source. [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sigL.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10748]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Binding to promoters of the -12, -24 type
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| * '''Protein family:''' sigma-54 factor family (according to Swiss-Prot) sigma-54 factor family
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| * '''Paralogous protein(s):'''
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| === Genes/ operons controlled by SigL ===
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| ''[[acoA]]-[[acoB]]-[[acoC]]-[[acoL]]'', ''[[rocA]]-[[rocB]]-[[rocC]]'', ''[[rocD]]-[[rocE]]-[[rocF]]'', ''[[rocG]]'', ''[[levD]]-[[levE]]-[[levF]]-[[levG]]-[[sacC]]'', ''[[ptb]]-[[bcd]]-[[buk]]-[[lpdV]]-[[bkdAA]]-[[bkdAB]]-[[bkdB]]''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| ** DNA binding domain (H-T-H motif) (324–343)
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| ** pron box domain (413–421)
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| ** 3 x Compositional bias domain (6–21),(32–53),(112–136)
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' [[SigL]]-[[RocR]], [[SigL]]-[[LevR]], [[SigL]]-[[BkdR]], [[SigL]]-[[YplP]], SigL-[[RpoB]]-[[RpoC]]
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P24219 P24219]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU34200]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:''' repressed by glucose ([[CcpA]]) , neg. regulated by [[CcpA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+16166551 PubMed]
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| * '''Regulatory mechanism:''' [[CcpA]]: transcription repression, transcriptional roadblock
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP146 (aphA3), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Michel Debarbouille]], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>16585774,11274109,, </pubmed>
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| [[Category:Protein-coding genes]]
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