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| * '''Description:''' penicillin-binding protein I, D-alanyl-D-alanine carboxypeptidase <br/><br/>
| | {{#bank: DacF}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''dacF''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || penicillin-binding protein I, <br/>D-alanyl-D-alanine carboxypeptidase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || carboxypeptidase
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 43 kDa, 9.912
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1167 bp, 389 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoIIAA]]'', ''[[pupG]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14280]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:dacF_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU23480
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacF-spoIIAAB-sigF.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10295]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)
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| * '''Protein family:''' peptidase S11 family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P38422 P38422]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU23480]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]''
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| * '''[[Sigma factor]]:''' [[SigF]], [[SigG]]
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| * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] , expressed during sporulation in the prespore [http://www.ncbi.nlm.nih.gov/sites/entrez/15758244 Link to a summary]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>16497325,8936302,,9864321,12107147, </pubmed>
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