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| * '''Description:''' required for SigG activation <br/><br/>
| | {{#bank: SpoIIIAH}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''spoIIIAH''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || unknown
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| |style="background:#ABCDEF;" align="center"|'''Function''' || activation of SigG
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 4.627
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 654 bp, 218 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[accB]]'', ''[[spoIIIAG]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14367]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:spoIIIAH_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU24360
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yqhV-spoIIIAABCDEFGH.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11415]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' [[SpoIIIAH]]-[[SpoIIQ]]
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| * '''Localization:''' membrane protein [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P49785 P49785]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU24360]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[spoIIIAA]]-[[spoIIIAB]]-[[spoIIIAC]]-[[spoIIIAD]]-[[spoIIIAE]]-[[spoIIIAF]]-[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
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| ** ''[[spoIIIAG]]-[[spoIIIAH]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
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| * '''[[Sigma factor]]:'''
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| ** both promoters: [[SigE]] [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed]
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| * '''Regulation:'''
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| ** both promoters: expressed during sporulation in the mother cell ([[SigE]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/18485064 PubMed], switched off by [[SpoIIID]] [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
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| * '''Regulatory mechanism:''' [[SpoIIID]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15383836 PubMed]
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| * '''Additional information:''' the internal promoter is essential for sporulation, it is twice as active as the promoter in front of [[spoIIIAA]], suggesting that [[SpoIIIAG]] and [[SpoIIIAH]] are required in larger amounts as compared to the other products of the operon [http://www.ncbi.nlm.nih.gov/sites/entrez/17693505 PubMed]
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| * [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>15752199,18485064,15574594,18812514,1766372,17693505,, </pubmed>
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