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| * '''Description:''' phosphofructokinase, glycolytic enzyme <br/><br/>
| | {{#bank: PfkA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pfkA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pfk''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes
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| |style="background:#ABCDEF;" align="center"| '''Product''' || 6-phosphofructokinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || catabolic enzyme in glycolysis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 34,1 kDa, 6.14
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 957 bp, 319 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[accA]]'', ''[[pyk]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14879]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pfkA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU29190
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| ===Phenotypes of a mutant ===
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| *Essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pfkA-pyk-ytzA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12644]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate (according to Swiss-Prot)
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| * '''Protein family:''' phosphofructokinase family (according to Swiss-Prot) phosphofructokinase family
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Allosteric Regulation (Reversible) [http://www.ncbi.nlm.nih.gov/pubmed/4269800 PubMed]
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| * '''Domains:'''
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| ** 3 x nucleotide binding domain (ATP) (21–25), (154–158), (171–187)
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| * '''Modification:'''
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| * '''Cofactor(s):''' Mg2+
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| * '''Effectors of protein activity:'''
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| ** Inhibited by citrate, PEP (Hill Coefficient 3) and Ca2+ (competes with Mg2+) in ''B. licheniformes'' [http://www.ncbi.nlm.nih.gov/pubmed/4269800 PubMed].
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| ** Inhibited by ATP (competitively) and f6p (non-competitively) in ''G. stearothermophillus'' [http://www.ncbi.nlm.nih.gov/pubmed/8136379 PubMed]
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| ** Activated by GDP and ADP in lower concentrations (1mM); above that inhibition, competing with the ATP for the binding site (in ''G. stearothermophillus'') [http://www.ncbi.nlm.nih.gov/pubmed/7873536 PubMed]
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| ** Activated by NH4+ [http://www.ncbi.nlm.nih.gov/pubmed/7873536 PubMed]
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| * '''Interactions:''' [[PfkA]]-[[Pgm]], [[PfkA]]-[[Eno]], [[PfkA]]-[[Rny]], [[PfkA]]-[[PnpA]], [[PfkA]]-[[RnjA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/19193632 PubMed]
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| * '''Localization:''' cytoplasm (according to Swiss-Prot), Cytoplasm (Homogeneous) [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1MTO 1MTO] (mutant, complex with fructose-6-phosphate, Geobacillus stearothermophilus), [http://www.rcsb.org/pdb/explore.do?structureId=4PFK 4PFK] (Geobacillus stearothermophilus), ''Geobacillus stearothermophilus'' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=58449 NCBI], Mutant form, complex with fructose-6-phosphate ''Geobacillus stearothermophilus'' [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=21480 NCBI]
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34529 O34529]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29190]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.11 2.7.1.11]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''pfkA [[pyk]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11489127 PubMed]
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| * '''Sigma factor:'''
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| * '''Regulation:''' twofold induced by glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/11489127 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:''' pGP393 (N-terminal His-tag, in [[pWH844]]), pGP87 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]]), available in [[Stülke]] lab
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| * '''lacZ fusion:''' pGP511 (in [[pAC6]]), available in [[Stülke]] lab
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed> 16479537,19193632, 11489127, 8136379, 7873536 4269800 </pubmed>
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