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| * '''Description:''' fructose 1,6-bisphosphate aldolase, glycolytic/ gluconeogenic enzyme<br/><br/>
| | {{#bank: FbaA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''fbaA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''fba, fba1, tsr ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || yes
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| |style="background:#ABCDEF;" align="center"| '''Product''' || fructose-1,6-bisphosphate aldolase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || enzyme in glycolysis/ gluconeogenesis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism], [http://subtiwiki.uni-goettingen.de/pathways/carbohydrate_metabolic_pathways.html Sugar catabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 30,2 kDa, 5.03
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 855 bp, 285 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spo0F]]'', ''[[ywjH]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB15729]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:fbaA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU37120
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| ===Phenotypes of a mutant ===
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| *Essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fbaA-ywjH.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10412]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate (according to Swiss-Prot)
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| * '''Protein family:''' class II fructose-bisphosphate aldolase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[FbaB]]
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Reversible Michaelis-Menten [http://www.ncbi.nlm.nih.gov/sites/entrez/15125960 PubMed]
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| * '''Domains:'''
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| ** 2 x Dihydroxyacetone phosphate binding domain (210–212), (231–234)
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| * '''Modification:''' phosphorylation on Thr-212 and Thr-234 [http://www.ncbi.nlm.nih.gov/sites/entrez/17218307 PubMed]
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| * '''Cofactor(s):''' Zn2+ (Metalloenzyme)
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| * '''Effectors of protein activity:'''
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| ** Inhibited by alpha-ketoglutarate, oxaloacetate and pyruvate [http://www.ncbi.nlm.nih.gov/pubmed/24624 PubMed] [http://www.ncbi.nlm.nih.gov/sites/entrez/15125960 PubMed]
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| ** Activated by NH4+ [http://www.ncbi.nlm.nih.gov/sites/entrez/15125960 PubMed]
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P13243 P13243]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU37120]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/4.1.2.13 4.1.2.13]
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| === Additional information===
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| Binds 2 zinc ions per subunit. One is catalytic and the other provides a structural contribution
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| =Expression and regulation=
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| * '''Operon:'''
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| **''fbaA'' [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]
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| **''fbaA-[[ywjH]]'' [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]
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| * '''Sigma factor:'''
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| * '''Regulation:''' constitutively expressed [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+11489127 PubMed]
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:''' pGP395 (N-terminal His-tag, in [[pWH844]]), pGP88 (N-terminal Strep-tag, for [[SPINE]], expression in ''B. subtilis'', in [[pGP380]])
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| * '''lacZ fusion:''' pGP601 (in [[pAC6]])
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>17218307, 15125960, 24624 </pubmed>
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