|
|
| (34 intermediate revisions by 5 users not shown) |
| Line 1: |
Line 1: |
| * '''Description:''' ATP-dependent deoxyribonuclease (subunit B) <br/><br/>
| | {{#bank: AddB}} |
|
| |
|
| {| align="right" border="1" cellpadding="2"
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Gene name'''
| |
| |''addB''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Product''' || ATP-dependent deoxyribonuclease (subunit B))
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Function''' || DNA repair and recombination
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 134 kDa, 5.39
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 3498 bp, 1166 aa
| |
| |-
| |
| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yhjR]]'', ''[[addA]]''
| |
| |-
| |
| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12902]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
| |
| |-
| |
| |-
| |
| |-
| |
| |colspan="2" | '''Genetic context''' <br/> [[Image:addB_context.gif]]
| |
| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
| |
| |-
| |
| |}
| |
|
| |
| __TOC__
| |
|
| |
| <br/><br/><br/><br/>
| |
|
| |
| =The gene=
| |
|
| |
| === Basic information ===
| |
|
| |
| * '''Locus tag:''' BSU10620
| |
|
| |
| ===Phenotypes of a mutant ===
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/addBA.html]
| |
|
| |
| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10465]
| |
|
| |
| === Additional information===
| |
|
| |
| =The protein=
| |
|
| |
| === Basic information/ Evolution ===
| |
|
| |
| * '''Catalyzed reaction/ biological activity:'''
| |
|
| |
| * '''Protein family:''' uvrD-like helicase C-terminal domain (according to Swiss-Prot)
| |
|
| |
| * '''Paralogous protein(s):'''
| |
|
| |
| === Extended information on the protein ===
| |
|
| |
| * '''Kinetic information:'''
| |
|
| |
| * '''Domains:'''
| |
|
| |
| * '''Modification:'''
| |
|
| |
| * '''Cofactor(s):'''
| |
|
| |
| * '''Effectors of protein activity:'''
| |
|
| |
| * '''Interactions:''' [[AddA]]-[[AddB]] {{PubMed|10756102}}
| |
|
| |
| * '''Localization:'''
| |
|
| |
| === Database entries ===
| |
|
| |
| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1Y26 1Y26] (the [[riboswitch]] in complex with adenine)
| |
|
| |
| * '''UniProt:''' [http://www.uniprot.org/uniprot/P23477 P23477]
| |
|
| |
| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU10620]
| |
|
| |
| * '''E.C. number:'''
| |
|
| |
| === Additional information===
| |
|
| |
| =Expression and regulation=
| |
|
| |
| * '''Operon:''' ''[[addB]]-[[addA]]'' {{PubMed|1646786}}
| |
|
| |
| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7746142}}
| |
|
| |
| * '''Regulation:'''
| |
| ** positive control by [[ComK]] {{PubMed|7746142}}
| |
|
| |
| * '''Regulatory mechanism:'''
| |
|
| |
| * '''Additional information:'''
| |
|
| |
| =Biological materials =
| |
|
| |
| * '''Mutant:''' GP1106 (addAB, spc), available in [[Stülke]] lab
| |
|
| |
| * '''Expression vector:'''
| |
|
| |
| * '''lacZ fusion:'''
| |
|
| |
| * '''GFP fusion:'''
| |
|
| |
| * '''two-hybrid system:'''
| |
|
| |
| * '''Antibody:'''
| |
|
| |
| =Labs working on this gene/protein=
| |
|
| |
| =Your additional remarks=
| |
|
| |
| =References=
| |
| ==Reviews==
| |
| <pubmed>, 20116346 </pubmed>
| |
| ==Original publications==
| |
| <pubmed>,8387145,15610857,7746142, 19129187 1646786 10756102 9781875 17570399 20350930 </pubmed>
| |
|
| |
| [[Category:Protein-coding genes]]
| |
