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| * '''Description:''' pyruvate carboxylase <br/><br/>
| | {{#bank: PycA}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pycA''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylaP ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || pyruvate carboxylase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || replenishment of the oxaloacetate pool
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 127 kDa, 5.407
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 3444 bp, 1148 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ftsW]]'', ''[[ctaA]]''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pycA_nucleotide.txt Gene sequence (+200bp) ]'''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pycA_protein.txt Protein sequence]'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pycA_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |-
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:'''
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ftsW-pycA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12660]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:''' membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=3BG5 3BG5] (''S. aureus'')
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| * '''Swiss prot entry:'''
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU14860]
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| * '''E.C. number:''' 6.4.1.1
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| === Additional information===
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| PycA binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins.
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| PycA is subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| =Expression and regulation=
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| * '''Operon:''' ''[[ftsW]]''-''[[pycA]]''
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:''' subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| # Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing ''Bacillus subtilis'' by divergent approaches. Proteomics '''8:''' 4123-4136 [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]# Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing ''Bacillus subtilis'' by divergent approaches. Proteomics 8: 4123-4136 [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
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| # Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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