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| * '''Description:''' chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by [[CheR]], enhances phosphatase activity of [[CheC]], required for full [[McpC]] activity <br/><br/>
| | {{#bank: CheD}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cheD''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ylxK ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || protein deaminase, [[CheC]] activity modulator
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| |style="background:#ABCDEF;" align="center"|'''Function''' || [[motility and chemotaxis]]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16460 cheD]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CheD CheD]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 9.347
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 498 bp, 166 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cheC]]'', ''[[sigD]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU16460 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU16460 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU16460 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:cheD_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheD_1715970_1716470_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cheD_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU16460]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[protein modification]]}},
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| {{SubtiWiki category|[[motility and chemotaxis]]}}
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|
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[SigD regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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|
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16460
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16460&redirect=T BSU16460]
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10750]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| ** binds chemoreceptors and increases their ability to activate the kinase activity of [[CheA]] {{PubMed|23226535}}
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| ** Protein L-glutamine + H<sub>2</sub>O = protein L-glutamate + NH<sub>3</sub> (according to Swiss-Prot)
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| ** deaminates Gln-586, Gln-593, and Gln594 in [[McpA]] {{PubMed|22931217}}
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| ** deaminates Gln-609 in [[McpC]] {{PubMed|22931217}}
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| * '''Protein family:''' cheD family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| ** [[CheC]]-[[CheD]] {{PubMed|17908686}}, this interaction is enhanced by [[CheY]]-P {{PubMed|23226535}}
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| ** [[CheD]]-[[McpA]], deamination of Gln-586, Gln-593 and Gln-594 in [[McpA]] {{PubMed|22931217}}
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| ** [[CheD]]-[[McpC]], deamination of Gln-609 in [[McpC]] {{PubMed|22931217}}
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU16460&redirect=T BSU16460]
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P40404 P40404]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16460]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/3.5.1.44 3.5.1.44]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
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| ** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheD_1715970_1716470_1 cheD] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| ** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
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| ** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
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| * '''Regulation:'''
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|14651647}}
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription repression {{PubMed|14651647}}
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| ** [[CodY]]: transcription repression {{PubMed|12730172}}
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| ** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}}
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| * '''Additional information:'''
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| ** in minimal medium, CheD is present with 1,200 +/- 250 molecules per cell {{PubMed|21515776}}
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| =Biological materials =
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| * '''Mutant:'''
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| ** 1A861 ( ''cheD''::''cat''), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A861&Search=1A861 BGSC]
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| ** 1A863 ( ''cheD''::''cat''), {{PubMed|7893679}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A863&Search=1A863 BGSC]
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed>,18774298,,18990184</pubmed>
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| ==Original publications==
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| <pubmed>17850253 21515776</pubmed>
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| ___
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| <pubmed>22931217 23226535</pubmed>
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| ____
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| <pubmed>7893679,15317802,17908686,12011078,8866475,11722727,9194713,14651647,9657996,8157612,15175317,16469702</pubmed>
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| [[Category:Protein-coding genes]]
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