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| * '''Description:''' modulation of [[CheA]] activity in response to attractants, scaffold protein: facilitates coupling between [[CheA]] and receptors <br/><br/>
| | {{#bank: CheW}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''cheW''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[CheA]] modulator
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| |style="background:#ABCDEF;" align="center"|'''Function''' || control of [[CheA]] activity
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 17 kDa, 4.422
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 468 bp, 156 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[cheA]]'', ''[[cheC]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13517]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:cheW_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheW_1714855_1715325_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:cheW_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[motility and chemotaxis]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[CodY regulon]]}},
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| {{SubtiWiki regulon|[[SigD regulon]]}},
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| {{SubtiWiki regulon|[[Spo0A regulon]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU16440
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| ===Phenotypes of a mutant ===
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| * ''[[cheV]] [[cheW]]'' double mutants exhibit complete loss of chemotaxis {{PubMed|21098025}}
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10692]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:'''
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| * '''Protein family:'''
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| * '''Paralogous protein(s):''' [[CheV]] (N-terminal domain)
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** cytoplasm (according to Swiss-Prot)
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| ** predominantly present at the cell poles {{PubMed|21098025}}
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39802 P39802]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16440]
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| * '''E.C. number:'''
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| === Additional information===
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| :* subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| =Expression and regulation=
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| * '''Operon:'''
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| ** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[ylxH]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
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| ** ''[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[ylxH]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|20233303}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cheW_1714855_1715325_1 cheW] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| ** ''[[flgB]]'': [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
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| ** ''[[ylxF]]'': [[SigD]] {{PubMed|20233303}}
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| * '''Regulation:'''
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| ** repressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|14651647}}
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| * '''Regulatory mechanism:'''
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| ** [[Spo0A]]: transcription repression {{PubMed|14651647}}
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| ** [[CodY]]: transcription repression {{PubMed|12730172}}
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| ** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}}
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| * '''Additional information:'''
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| ** subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| ** in minimal medium, CheW is present with 2,100 +/- 430 molecules per cell {{PubMed|21515776}}
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| ==Reviews==
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| <pubmed> 20122866 </pubmed>
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| ==Original publications==
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| <pubmed>,8169224,1601874,14731274,1624415,14651647, 9657996,8157612,15175317 17850253 21098025 21515776</pubmed>
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| [[Category:Protein-coding genes]]
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