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| * '''Description:''' glutamate 5-kinase <br/><br/>
| | {{#bank: ProB}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''proB''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || glutamate 5-kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || biosynthesis of proline
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/proline.html Pro], [http://subtiwiki.uni-goettingen.de/pathways/stress_response.html Stress]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 39 kDa, 6.01
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1095 bp, 365 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykkE]]'', ''[[proA]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13169]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:proB_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[biosynthesis/ acquisition of amino acids]]}}
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| = This gene is a member of the following [[regulons]] =
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| {{SubtiWiki regulon|[[T-box]]}}
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU13120
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| [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=proB_1378496_1379593_1 Expression]
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| ===Phenotypes of a mutant ===
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| * auxotrophic for proline {{PubMed|21784929}}
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10963]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + L-glutamate = ADP + L-glutamate 5-phosphate (according to Swiss-Prot)
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| * '''Protein family:''' PUA domain (according to Swiss-Prot)
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| * '''Paralogous protein(s):''' [[ProJ]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:''' the enzymatic activity is feedback-inhibited by proline {PubMed|16958849}}
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| ** cytoplasm (according to Swiss-Prot)
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P39820 P39820]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU13120]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.2.11 2.7.2.11]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[proB]]-[[proA]]''{{PubMed|21233158}}
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| * '''[[Sigma factor]]:''' [[SigA]] {{PubMed|21233158}}
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| * '''Regulation:'''
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| ** induced by proline limitation ([[T-box]]) {{PubMed|21233158}}
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| * '''Regulatory mechanism:'''
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| ** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|21233158}}
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| '''Additional publications:''' {{PubMed|21784929}}
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| <pubmed>19258532, 16958849, 17562291 21233158 </pubmed>
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| [[Category:Protein-coding genes]]
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