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| * '''Description:''' pyruvate kinase, glycolytic enzyme <br/><br/>
| | {{#bank: Pyk}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pyk''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pykA''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || pyruvate kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || catabolic enzyme in glycolysis
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU29180 pyk]
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/carbon_flow.html Central C-metabolism]'''
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 61,9 kDa, 4.88
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1755 bp, 585 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ytzA]]'', ''[[pfkA]]''
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| |style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU29180 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU29180 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU29180 DNA_with_flanks]
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pyk_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:pyk_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/><br/><br/>
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[ATP synthesis]]}},
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| {{SubtiWiki category|[[carbon core metabolism]]}},
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| {{SubtiWiki category|[[phosphoproteins]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU29180
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| ===Phenotypes of a mutant ===
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| Unable to grow with non-[[PTS]] carbohydrates (such as glucitol or glycerol) as single carbon source.
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pfkA-pyk-ytzA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12661]
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| === Additional information===
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| * A mutation was found in this gene after evolution under relaxed selection for sporulation {{PubMed|21821766}}
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ADP + phosphoenolpyruvate --> ATP + pyruvate
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| ** The reaction is irreversible under physiological conditions
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| * '''Protein family:''' PEP-utilizing enzyme family (according to Swiss-Prot) pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:''' Allosteric Regulation [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]
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| * '''Domains:'''
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| * '''Modification:''' phosphorylation on Ser36 [http://www.ncbi.nlm.nih.gov/pubmed/17218307 PubMed], [http://www.ncbi.nlm.nih.gov/pubmed/16493705 PubMed], phosphorylation on Ser536 or Ser546 [http://www.ncbi.nlm.nih.gov/pubmed/17726680 PubMed], please note that the Ser is not on position 536 but rather at 538
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| * '''Cofactor(s):''' Mg2+, K+
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| * '''Effectors of protein activity:'''
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| ** Activated by PEP (Hill Coefficient 1,8) [http://www.ncbi.nlm.nih.gov/sites/entrez/4623707 PubMed] [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]
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| ** Allosterically activated by AMP [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]
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| ** Activation by r5p and ADP [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]
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| ** Inhibition by ADP and f16bp in high concentrations; and ATP [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:''' cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2E28 2E28] (Geobacillus stearothermophilus)
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/P80885 P80885]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29180]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.40 2.7.1.40]
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| === Additional information===
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| The enzyme is a tetramer with four active sites [http://www.ncbi.nlm.nih.gov/pubmed/3711058 PubMed]
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| =Expression and regulation=
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| * '''Operon:''' ''[[pfkA]]-[[pyk]]-[[ytzA]]'' {{PubMed|11489127}}
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyk_2984788_2986545_-1 pyk] {{PubMed|22383849}}
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| * '''Sigma factor:'''
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| * '''Regulation:'''
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| ** twofold induced by glucose {{PubMed|11489127}}
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * GP589 (''pyk''::''cat''), available in [[Stülke]] lab
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| * GP600 (''pyk''::''erm''), available in [[Stülke]] lab
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| * '''Expression vector:'''
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| ** expression in ''E. coli'', N-terminal His-tag: pGP1100 (in [[pWH844]]), available in [[Stülke]] lab
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| ** expression in ''B. subtilis'', native protein: pGP1411 (in [[pBQ200]]), available in [[Stülke]] lab
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| ** expression in ''B. subtilis'', N-terminal Strep-tag: pGP1409 (in [[pGP380]]), available in [[Stülke]] lab
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| ** expression in ''B. subtilis'', C-terminal Strep-tag: pGP1410 (in [[pGP382]]), available in [[Stülke]] lab
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| * '''lacZ fusion:''' see ''[[pfkA]]''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:''' ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Stülke]] lab
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| [[Stülke|Jörg Stülke]], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| =Your additional remarks=
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| =References=
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| <pubmed>17726680 16493705 11489127 17505547 10966427 17218307 3711058 4623707 21821766 22846916 </pubmed>
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| [[Category:Protein-coding genes]]
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