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| * '''Description:''' catalyzes the transfere of a phosphate from phosphoennolpyruvate to ADT and synthesis of pyruvate <br/><br/>
| | {{#bank: Pyk}} |
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''pyk''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''pykA''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || Pyruvate kinase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || kinase, transferase
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 61,9 kDa, 4.88
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1755 bp, 585 amino acids
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pfkA]]'', ''[[ytzA]]''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pyk_nucleotide.txt Gene sequence (+200bp) ]'''
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| |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/pyk_protein.txt Protein sequence]'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:pyk_context.gif]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Coordinates:''' 2983830 - 2985584
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pfkA-pyk-ytzA.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12661]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' ATP + pyruvate = ADP + phosphoenolpyruvate
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| * '''Protein family:''' pyruvate kinase family, (C-terminal section: PEP-utilizing enzyme family)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:''' phosphorylation
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| * '''Cofactor(s):''' magnesium ion, potassium
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| * '''Effectors of protein activity:'''
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| * '''Interactions:''' Pyk-[[PfkA]]
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| * '''Localization:''' cytoplasm [http://www.ncbi.nlm.nih.gov/sites/entrez/16479537 PubMed]
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| === Database entries ===
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| * '''Structure:'''
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P80885]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU29180]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.40]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''[[pfkA]] [[pyk]]''
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| * '''Sigma factor:'''
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| * '''Regulation:''' twofold induced by glucose
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| =Labs working on this gene/protein=
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| [[Stülke|Jörg Stülke]], University of Göttingen, Germany
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| [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
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| =Your additional remarks=
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| =References=
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| # Ludwig, H., Homuth, G., Schmalisch, M., Dyka, F. M., Hecker, M., and Stülke, J. (2001) Transcription of glycolytic genes and operons in ''Bacillus subtilis'': evidence for the presence of multiple levels of control of the ''gapA'' operon. Mol Microbiol 41, 409-422.[http://www.ncbi.nlm.nih.gov/sites/entrez/11489127 PubMed]
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