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| * '''Description:''' D-alanine aminotransferase
| | {{#bank: Dat}} |
| <small>(Another gene coding for O6-methylguanine DNA alkyltransferase was formely known as dat, it is now called [[ogt]])</small><br/><br/>
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| {| align="right" border="1" cellpadding="2"
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''dat''
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yheM ''
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || D-alanine aminotransferase
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| |style="background:#ABCDEF;" align="center"|'''Function''' || peptidoglycan precursor biosynthesis
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 31 kDa, 4.871
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 846 bp, 282 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yheN]]'', ''[[nhaC]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12806]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:dat_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |}
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| __TOC__
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| <br/><br/>
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU09670
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| ===Phenotypes of a mutant ===
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| === Database entries ===
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| * '''DBTBS entry:''' no entry
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13045]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' D-alanine + 2-oxoglutarate = pyruvate + D-glutamate (according to Swiss-Prot)
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| * '''Protein family:''' class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
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| * '''Paralogous protein(s):'''
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''Interactions:'''
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| * '''Localization:'''
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| === Database entries ===
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| * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1G2W 1G2W] (E177S Mutant, Geobacillus stearothermophilus, 43% identity)
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| * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O07597 O07597]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU09670]
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| * '''E.C. number:''' [http://www.expasy.org/enzyme/2.6.1.21 2.6.1.21]
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:'''
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:'''
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| * '''Expression vector:'''
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| * '''two-hybrid system:'''
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
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