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| * '''Description:''' [[DEAD-box RNA helicases|DEAD-box RNA helicase]]<br/><br/>
| | {{#bank: YfmL}} |
| {| align="right" border="1" cellpadding="2" | |
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| |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| |''yfmL''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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| |-
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| |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| |style="background:#ABCDEF;" align="center"| '''Product''' || [[DEAD-box RNA helicases|DEAD-box RNA helicase]]
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| |style="background:#ABCDEF;" align="center"|'''Function''' || RNA helicase
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| |colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://cellpublisher.gobics.de/subtiexpress/ ''Subti''Express]''': [http://cellpublisher.gobics.de/subtiexpress/bsu/BSU07430 yfmL]
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| |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 42 kDa, 9.602
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| |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1128 bp, 376 aa
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| |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yfmM]]'', ''[[yfmK]]''
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| |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB12572]+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
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| |colspan="2" | '''Genetic context''' <br/> [[Image:yfmL_context.gif]]
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| <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
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| |colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfmL_816113_817243_1 Expression at a glance]'''   {{PubMed|22383849}}<br/>[[Image:yfmL_expression.png|500px]]
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| |}
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| __TOC__
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/><br/><br/>
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| <br/><br/>
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|
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| = [[Categories]] containing this gene/protein =
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| {{SubtiWiki category|[[DEAD-box RNA helicases]]}},
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| {{SubtiWiki category|[[translation]]}}
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| = This gene is a member of the following [[regulons]] =
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| =The gene=
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| === Basic information ===
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| * '''Locus tag:''' BSU07430
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| ===Phenotypes of a mutant ===
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| * poor growth at low temperatures (16 to 20°C) {{PubMed|23175651}}
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| === Database entries ===
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| * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yfmL.html]
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| * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12963]
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| === Additional information===
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| =The protein=
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| === Basic information/ Evolution ===
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| * '''Catalyzed reaction/ biological activity:''' RNA helicase
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| * '''Protein family:''' helicase C-terminal domain (according to Swiss-Prot) [[DEAD-box RNA helicases|DEAD-box RNA helicase]]
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| * '''Paralogous protein(s):''' [[CshA]], [[CshB]], [[DeaD]]
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| === Extended information on the protein ===
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| * '''Kinetic information:'''
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| * '''Domains:'''
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| * '''Modification:'''
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| * '''Cofactor(s):'''
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| * '''Effectors of protein activity:'''
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| * '''[[SubtInteract|Interactions]]:'''
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| * '''[[Localization]]:'''
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| === Database entries ===
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| * '''Structure:'''
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| * '''UniProt:''' [http://www.uniprot.org/uniprot/O34750 O34750]
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| * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU07430]
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| * '''E.C. number:'''
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| === Additional information===
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| =Expression and regulation=
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| * '''Operon:''' ''yfmL'' (according to [http://dbtbs.hgc.jp/COG/prom/yfmL.html DBTBS])
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| * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yfmL_816113_817243_1 yfmL] {{PubMed|22383849}}
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| * '''[[Sigma factor]]:'''
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| * '''Regulation:'''
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| * '''Regulatory mechanism:'''
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| * '''Additional information:'''
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| =Biological materials =
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| * '''Mutant:''' GP1053 (erm), available in the [[Stülke]] lab
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| * '''Expression vector:'''
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| ** for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [[SPINE]], expression from the native chromomsomal site: GP1069 (spc), available in [[Jörg Stülke]]'s lab
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| * '''lacZ fusion:'''
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| * '''GFP fusion:'''
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| ** B. subtilis GP1082 yfmL-yfp spc, available in [[Jörg Stülke]]'s lab
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| * '''two-hybrid system:''' B. pertussis adenylate cyclase-based bacterial two hybrid system ([[BACTH]]), available in [[Jörg Stülke]]'s lab
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| * '''FLAG-tag construct:'''
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| ** GP1066 (spc, based on [[pGP1331]]), available in [[Jörg Stülke]]'s
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| * '''Antibody:'''
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| =Labs working on this gene/protein=
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| =Your additional remarks=
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| =References=
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| <pubmed>23175651</pubmed>
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| [[Category:Protein-coding genes]]
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